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Use of Commercially Available Rabbit Monoclonal Antibodies for Immunofluorescence Double Staining

Use of Commercially Available Rabbit Monoclonal Antibodies for Immunofluorescence Double Staining Downloaded from http://journals.lww.com/appliedimmunohist by BhDMf5ePHKbH4TTImqenVA5KvPVPZ0P5BEgU+IUTEfzO/GUWifn2IfwcEVVH9SSn on 06/10/2020 TECHNICAL ARTICLE Use of Commercially Available Rabbit Monoclonal Antibodies for Immunofluorescence Double Staining Michael Bzorek, MT,* Inger Merete Stamp, MD,* Bodil Laub Petersen, MD, PhD,w and Lisbeth Frederiksen, MT* reactivity between immunoreagents detecting the first and 7–10 Abstract: Immunohistochemistry, that is, the use of polyclonal second antigen of interest. Second, using double and monoclonal antibodies to detect cell and tissue antigens at a immunoenzymatic technique often requires extensive microscopical level is a powerful tool for both research and laboratory work and control experiments to obtain diagnostic purposes. Especially in the field of hematologic colocalized signal, especially with suboptimal chromogen 11–13 disease, there is often a need to detect several antigens combinations in the detection step. synchronously, and we report here a fast and easy technique Previously, Mason et al described a double for demonstrating more than 1 antigen in 1 slide using immunofluorescence labeling technique applied to routi- immunofluorescence. We have used commercially available nely processed paraffin sections. Inspired by this work rabbit monoclonal antibodies (Cyclin D1, CD3, CD5, CD23, and with the development of new rabbit monoclonal etc.) paired with mouse monoclonal antibodies (CD7, CD20, antibodies (RMAs) (eg, Cyclin D1 clone http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied Immunohistochemistry & Molecular Morphology Wolters Kluwer Health

Use of Commercially Available Rabbit Monoclonal Antibodies for Immunofluorescence Double Staining

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References (32)

Publisher
Wolters Kluwer Health
ISSN
1541-2016
DOI
10.1097/PAI.0b013e3181594ec6
Publisher site
See Article on Publisher Site

Abstract

Downloaded from http://journals.lww.com/appliedimmunohist by BhDMf5ePHKbH4TTImqenVA5KvPVPZ0P5BEgU+IUTEfzO/GUWifn2IfwcEVVH9SSn on 06/10/2020 TECHNICAL ARTICLE Use of Commercially Available Rabbit Monoclonal Antibodies for Immunofluorescence Double Staining Michael Bzorek, MT,* Inger Merete Stamp, MD,* Bodil Laub Petersen, MD, PhD,w and Lisbeth Frederiksen, MT* reactivity between immunoreagents detecting the first and 7–10 Abstract: Immunohistochemistry, that is, the use of polyclonal second antigen of interest. Second, using double and monoclonal antibodies to detect cell and tissue antigens at a immunoenzymatic technique often requires extensive microscopical level is a powerful tool for both research and laboratory work and control experiments to obtain diagnostic purposes. Especially in the field of hematologic colocalized signal, especially with suboptimal chromogen 11–13 disease, there is often a need to detect several antigens combinations in the detection step. synchronously, and we report here a fast and easy technique Previously, Mason et al described a double for demonstrating more than 1 antigen in 1 slide using immunofluorescence labeling technique applied to routi- immunofluorescence. We have used commercially available nely processed paraffin sections. Inspired by this work rabbit monoclonal antibodies (Cyclin D1, CD3, CD5, CD23, and with the development of new rabbit monoclonal etc.) paired with mouse monoclonal antibodies (CD7, CD20, antibodies (RMAs) (eg, Cyclin D1 clone

Journal

Applied Immunohistochemistry & Molecular Morphology – Wolters Kluwer Health

Published: Jul 1, 2008

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