Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Uptake of Low Density Lipoprotein, Albumin, and Water by Deendothelialized In Vitro Minipig Aorta

Uptake of Low Density Lipoprotein, Albumin, and Water by Deendothelialized In Vitro... The purpose of this research was to study the effect of pressure on arterial hydration in vitro and the effect of pressure and flow (stirred reagent) on the in vitro transport of 125 l-albumin and 125l-LDL into deendothelialized minipig aortas over a 24-hour period. It was found that the arterial hydration (fractional mass of water) was 0.740 ± 0.0043 SEM for control tissue; after 24 hours this rose to 0.745 ±0.0038 for 0 mm Hg, 0.752 ± 0.0046 for 100 mm Hg, and 0.755 ±0.0065 for 200 mm Hg. In the transport studies, the following effect were found. 1) The reagent radioactivity concentration and composition did not change with pressure or stirring over the 24-hour period. 2) For unpressurized tissue, the 24-hour normalized uptake (uptake (M mg cm-2) ÷ reagent concentration (co mg cm-3)] of albumin was (4.86 ± 0.43 cm)× 10−3from stirred and (5.46 ± 0.36 cm) × 10−3from nonstitrred reagent; that of LDL was (0.31 ± 0.02 cm)× 10−3from stirred and (0.37 ± 0.02 cm)× 10−3from nonstirred reagent. 3) PRessurization (100 mm Hg) of the tissue increased albumin uptake by 52% from stirred and by 125% from nonstirred reagent and the LDL uptake by 52% from stirred and 241% from nonstirred reagent. 4) Pressure increased the intimal surface concentration of albumin and LDL at the nonstirred, but not at stirred, interfaces. 5) The electrophoretic properties of the intimal surface fluid showed only minor differences from thoseof the bulk reagent. These data demonstrate that pressure causes a slight, but significant, increase in arterial hydration and that radiolabeled albumin and LDL appear to be sieve by the superficial intimal layers of the deendothelialized porcine aorta under the in vitro conditions of this study. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Arteriosclerosis Wolters Kluwer Health

Uptake of Low Density Lipoprotein, Albumin, and Water by Deendothelialized In Vitro Minipig Aorta

Download PDF
16 pages

Loading next page...
 
/lp/wolters-kluwer-health/uptake-of-low-density-lipoprotein-comma-albumin-comma-and-water-by-lOHBK0040G

References

References for this paper are not available at this time. We will be adding them shortly, thank you for your patience.

Copyright
© 1986 by American Heart Association, Inc.
ISSN
0276-5047

Abstract

The purpose of this research was to study the effect of pressure on arterial hydration in vitro and the effect of pressure and flow (stirred reagent) on the in vitro transport of 125 l-albumin and 125l-LDL into deendothelialized minipig aortas over a 24-hour period. It was found that the arterial hydration (fractional mass of water) was 0.740 ± 0.0043 SEM for control tissue; after 24 hours this rose to 0.745 ±0.0038 for 0 mm Hg, 0.752 ± 0.0046 for 100 mm Hg, and 0.755 ±0.0065 for 200 mm Hg. In the transport studies, the following effect were found. 1) The reagent radioactivity concentration and composition did not change with pressure or stirring over the 24-hour period. 2) For unpressurized tissue, the 24-hour normalized uptake (uptake (M mg cm-2) ÷ reagent concentration (co mg cm-3)] of albumin was (4.86 ± 0.43 cm)× 10−3from stirred and (5.46 ± 0.36 cm) × 10−3from nonstitrred reagent; that of LDL was (0.31 ± 0.02 cm)× 10−3from stirred and (0.37 ± 0.02 cm)× 10−3from nonstirred reagent. 3) PRessurization (100 mm Hg) of the tissue increased albumin uptake by 52% from stirred and by 125% from nonstirred reagent and the LDL uptake by 52% from stirred and 241% from nonstirred reagent. 4) Pressure increased the intimal surface concentration of albumin and LDL at the nonstirred, but not at stirred, interfaces. 5) The electrophoretic properties of the intimal surface fluid showed only minor differences from thoseof the bulk reagent. These data demonstrate that pressure causes a slight, but significant, increase in arterial hydration and that radiolabeled albumin and LDL appear to be sieve by the superficial intimal layers of the deendothelialized porcine aorta under the in vitro conditions of this study.

Journal

Arteriosclerosis – Wolters Kluwer Health

Published: Sep 1, 1986

There are no references for this article.