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Translational Sciences Tissue-Specific RNA-Seq in Human Evoked Inflammation Identifies Blood and Adipose LincRNA Signatures of Cardiometabolic Diseases Yichuan Liu,* Jane F. Ferguson,* Chenyi Xue, Rachel L. Ballantyne, Ian M. Silverman, Sager J. Gosai, Jacquelyn Serfecz, Michael P. Morley, Brian D. Gregory, Mingyao Li,* Muredach P. Reilly* Objective—Inappropriate transcriptional activation of innate immunity is a pathological feature of several cardiometabolic disorders, but little is known about inflammatory modulation of long intergenic noncoding RNAs (lincRNAs) in disease- relevant human tissues. Approach and Results—We applied deep RNA sequencing (>500 million filtered reads per sample) to blood and adipose during low-dose experimental endotoxemia (lipopolysaccharide) in a healthy human, with targeted replication in separate individuals undergoing endotoxemia (n=6), to identify inflammatory lincRNAs. A subset of these lincRNAs was examined for expression in adipocytes and monocytes, modulation in adipose of obese humans, and overlap with genome-wide association study signals for inflammatory and cardiometabolic traits. Of a stringent set of 4284 lincRNAs, ≈11% to 22% were expressed with 201 and 56 lincRNAs modulated by lipopolysaccharide in blood or adipose, respectively. Tissue- specific expression of a subset of 6 lipopolysaccharide-lincRNAs was replicated with lipopolysaccharide modulation confirmed for all 3 expressed in blood and 2 of 4 expressed
Arteriosclerosis, Thrombosis, and Vascular Biology – Wolters Kluwer Health
Published: Apr 1, 2014
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