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The Role of Immunohistochemical Analysis in the Evaluation of EML4-ALK

The Role of Immunohistochemical Analysis in the Evaluation of EML4-ALK RESEARCH ARTICLE The Role of Immunohistochemical Analysis in the Evaluation of EML4-ALK Gene Rearrangement in Lung Cancer Harold C. Sullivan, MD, Kevin E. Fisher, MD, PhD, Anne L. Hoffa, MD, Jason Wang, MD, Debra Saxe, PhD, Momin T. Siddiqui, MD, and Cynthia Cohen, MD rearrangements. IHC shows promise as a screening tool to Background: Among the mutations described in non–small cell prevent unnecessary costly FISH analysis. lung carcinoma is a rearrangement resulting from an inversion Key Words: EML4-ALK, lung, adenocarcinoma, immunohisto- within chromosome 2p leading to the formation of a fusion gene, chemistry, fluorescence in situ hybridization echinoderm microtubule-associated protein-like 4-anaplastic lym- phoma kinase (EML4-ALK). Fluorescence in situ hybridization (Appl Immunohistochem Mol Morphol 2015;23:239–244) (FISH) is the gold standard for the detection of ALK gene re- arrangements. However, molecular methods are not readily avail- able in all pathology laboratories. Immunohistochemistry (IHC) number of genetic abnormalities have been described using an antibody directed against the EML4-ALK fusion protein Ain association with non–small cell lung carcinoma provides a widely available alternative method of detection. We (NSCLC). One such abnormality is a rearrangement re- assessed whether IHC is a comparable and cost-effective alternative sulting from a small inversion within the short arm http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied Immunohistochemistry & Molecular Morphology Wolters Kluwer Health

The Role of Immunohistochemical Analysis in the Evaluation of EML4-ALK

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Copyright © 2014 Wolters Kluwer Health, Inc. All rights reserved.
ISSN
1541-2016
eISSN
1533-4058

Abstract

RESEARCH ARTICLE The Role of Immunohistochemical Analysis in the Evaluation of EML4-ALK Gene Rearrangement in Lung Cancer Harold C. Sullivan, MD, Kevin E. Fisher, MD, PhD, Anne L. Hoffa, MD, Jason Wang, MD, Debra Saxe, PhD, Momin T. Siddiqui, MD, and Cynthia Cohen, MD rearrangements. IHC shows promise as a screening tool to Background: Among the mutations described in non–small cell prevent unnecessary costly FISH analysis. lung carcinoma is a rearrangement resulting from an inversion Key Words: EML4-ALK, lung, adenocarcinoma, immunohisto- within chromosome 2p leading to the formation of a fusion gene, chemistry, fluorescence in situ hybridization echinoderm microtubule-associated protein-like 4-anaplastic lym- phoma kinase (EML4-ALK). Fluorescence in situ hybridization (Appl Immunohistochem Mol Morphol 2015;23:239–244) (FISH) is the gold standard for the detection of ALK gene re- arrangements. However, molecular methods are not readily avail- able in all pathology laboratories. Immunohistochemistry (IHC) number of genetic abnormalities have been described using an antibody directed against the EML4-ALK fusion protein Ain association with non–small cell lung carcinoma provides a widely available alternative method of detection. We (NSCLC). One such abnormality is a rearrangement re- assessed whether IHC is a comparable and cost-effective alternative sulting from a small inversion within the short arm

Journal

Applied Immunohistochemistry & Molecular Morphology – Wolters Kluwer Health

Published: Apr 1, 2015

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