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Twenty-six of 44 human monoclonal IgM antibodies (MAbs) against erythrocyte blood group antigens were previously found to display unexpected cross-reactions with tissue components. The aim of the present study was to identify target antigens for these MAbs. Twenty of the 26 tissue-reactive subset reacted with intermediate filaments (IF) as shown by double-labelling immunofluorescence microscopy with mouse monoclonal antibodies against IF proteins; 14 of these MAbs also bound to purified IF proteins in solid-phase radiobinding assays. Four MAbs reacted with vimentin or glial fibrillary acidic protein (GFAP), whereas 16 showed more widespread reactivity with two or more classes of IF. Three MAbs bound to all five classes of IF, namely vimentin, GFAP, desmin, cytokeratins, and neurofilaments using immunohistochemistry or solid-phase radiobinding assays. Purified IF proteins were able to inhibit hemagglutination by IF-reactive MAbs. These results show a high incidence of cross-reactions with IF by a panel of antibodies produced from immunized donors, which has implications for the interpretation of immunohistochemical findings using antibodies produced from healthy and diseased persons.
Applied Immunohistochemistry & Molecular Morphology – Wolters Kluwer Health
Published: Jan 1, 1996
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