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The Basis of Unexpected Cross‐Reactions Shown by Human Monoclonal Antibodies Against Blood Group Antigens as Revealed by Immunohistochemistry

The Basis of Unexpected Cross‐Reactions Shown by Human Monoclonal Antibodies Against Blood... Twenty-six of 44 human monoclonal IgM antibodies (MAbs) against erythrocyte blood group antigens were previously found to display unexpected cross-reactions with tissue components. The aim of the present study was to identify target antigens for these MAbs. Twenty of the 26 tissue-reactive subset reacted with intermediate filaments (IF) as shown by double-labelling immunofluorescence microscopy with mouse monoclonal antibodies against IF proteins; 14 of these MAbs also bound to purified IF proteins in solid-phase radiobinding assays. Four MAbs reacted with vimentin or glial fibrillary acidic protein (GFAP), whereas 16 showed more widespread reactivity with two or more classes of IF. Three MAbs bound to all five classes of IF, namely vimentin, GFAP, desmin, cytokeratins, and neurofilaments using immunohistochemistry or solid-phase radiobinding assays. Purified IF proteins were able to inhibit hemagglutination by IF-reactive MAbs. These results show a high incidence of cross-reactions with IF by a panel of antibodies produced from immunized donors, which has implications for the interpretation of immunohistochemical findings using antibodies produced from healthy and diseased persons. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied Immunohistochemistry & Molecular Morphology Wolters Kluwer Health

The Basis of Unexpected Cross‐Reactions Shown by Human Monoclonal Antibodies Against Blood Group Antigens as Revealed by Immunohistochemistry

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ISSN
1062-3345
eISSN
1533-4058

Abstract

Twenty-six of 44 human monoclonal IgM antibodies (MAbs) against erythrocyte blood group antigens were previously found to display unexpected cross-reactions with tissue components. The aim of the present study was to identify target antigens for these MAbs. Twenty of the 26 tissue-reactive subset reacted with intermediate filaments (IF) as shown by double-labelling immunofluorescence microscopy with mouse monoclonal antibodies against IF proteins; 14 of these MAbs also bound to purified IF proteins in solid-phase radiobinding assays. Four MAbs reacted with vimentin or glial fibrillary acidic protein (GFAP), whereas 16 showed more widespread reactivity with two or more classes of IF. Three MAbs bound to all five classes of IF, namely vimentin, GFAP, desmin, cytokeratins, and neurofilaments using immunohistochemistry or solid-phase radiobinding assays. Purified IF proteins were able to inhibit hemagglutination by IF-reactive MAbs. These results show a high incidence of cross-reactions with IF by a panel of antibodies produced from immunized donors, which has implications for the interpretation of immunohistochemical findings using antibodies produced from healthy and diseased persons.

Journal

Applied Immunohistochemistry & Molecular MorphologyWolters Kluwer Health

Published: Jan 1, 1996

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