Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Standardization of Positive Controls in Diagnostic Immunohistochemistry Recommendations From the International Ad Hoc Expert Committee

Standardization of Positive Controls in Diagnostic Immunohistochemistry Recommendations From the... REVIEW ARTICLE Standardization of Positive Controls in Diagnostic Immunohistochemistry: Recommendations From the International Ad Hoc Expert Committee Emina E. Torlakovic, MD, PhD,*w Søren Nielsen, HT, CT,zy Glenn Francis, MBBS, FRCPA, MBA, FFSc (RCPA),8z# John Garratt, RT,w** Blake Gilks, MD, FRCPC,www Jeffrey D. Goldsmith, MD,zz Jason L. Hornick, MD, PhD,*yy Elizabeth Hyjek, MD, PhD,* Merdol Ibrahim, PhD,88 Keith Miller, FIBMS,88 Eugen Petcu, MD, PhD,8 Paul E. Swanson, MD,zz## Xiaoge Zhou, MD,***www Clive R. Taylor, MD, PhD,zzz and Mogens Vyberg, MDzy mittee has clarified definitions of IHC assay sensitivity and Abstract: Diagnostic immunohistochemistry (dIHC) has been specificity, with special emphasis on how these definitions apply practiced for several decades, with an ongoing expansion of to positive controls. Recommendations for “best laboratory applications for diagnostic use, and more recently for detection practice” regarding positive controls for dIHC are specified. The of prognostic and predictive biomarkers. However, stand- first set of immunohistochemistry critical assay performance ardization of practice has yet to be achieved, despite significant controls for several frequently used IHC stains or tests is also advances in methodology. An Ad Hoc Expert Committee was developed and presented. formed to address the standardization of controls, which is a Key Words: immunohistochemistry, standardization, positive http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied Immunohistochemistry & Molecular Morphology Wolters Kluwer Health

Standardization of Positive Controls in Diagnostic Immunohistochemistry Recommendations From the International Ad Hoc Expert Committee

Download PDF
18 pages

Loading next page...
 
/lp/wolters-kluwer-health/standardization-of-positive-controls-in-diagnostic-IOnexicCoh

References (66)

Copyright
Copyright © 2014 by Lippincott Williams & Wilkins
ISSN
1541-2016
eISSN
1533-4058
DOI
10.1097/PAI.0000000000000163
pmid
25474126
Publisher site
See Article on Publisher Site

Abstract

REVIEW ARTICLE Standardization of Positive Controls in Diagnostic Immunohistochemistry: Recommendations From the International Ad Hoc Expert Committee Emina E. Torlakovic, MD, PhD,*w Søren Nielsen, HT, CT,zy Glenn Francis, MBBS, FRCPA, MBA, FFSc (RCPA),8z# John Garratt, RT,w** Blake Gilks, MD, FRCPC,www Jeffrey D. Goldsmith, MD,zz Jason L. Hornick, MD, PhD,*yy Elizabeth Hyjek, MD, PhD,* Merdol Ibrahim, PhD,88 Keith Miller, FIBMS,88 Eugen Petcu, MD, PhD,8 Paul E. Swanson, MD,zz## Xiaoge Zhou, MD,***www Clive R. Taylor, MD, PhD,zzz and Mogens Vyberg, MDzy mittee has clarified definitions of IHC assay sensitivity and Abstract: Diagnostic immunohistochemistry (dIHC) has been specificity, with special emphasis on how these definitions apply practiced for several decades, with an ongoing expansion of to positive controls. Recommendations for “best laboratory applications for diagnostic use, and more recently for detection practice” regarding positive controls for dIHC are specified. The of prognostic and predictive biomarkers. However, stand- first set of immunohistochemistry critical assay performance ardization of practice has yet to be achieved, despite significant controls for several frequently used IHC stains or tests is also advances in methodology. An Ad Hoc Expert Committee was developed and presented. formed to address the standardization of controls, which is a Key Words: immunohistochemistry, standardization, positive

Journal

Applied Immunohistochemistry & Molecular Morphology – Wolters Kluwer Health

Published: Jan 1, 2015

There are no references for this article.