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Snap-Frozen Brain Tissue Sections Stored With Desiccant at Ambient Laboratory Conditions Without Chemical Fixation are Resistant to Degradation for a Minimum of 6 Months

Snap-Frozen Brain Tissue Sections Stored With Desiccant at Ambient Laboratory Conditions Without... TECHNICAL ARTICLE Snap-Frozen Brain Tissue Sections Stored With Desiccant at Ambient Laboratory Conditions Without Chemical Fixation are Resistant to Degradation for a Minimum of 6 Months Theodore R. Sadler, MS,*w Ani C. Khodavirdi, PhD,* David R. Hinton, MD,* and Daniel P. Holschneider, MDw zyJ them less compatible with current molecular techni- 1–3 Abstract: Cryosectioned tissues from snap-frozen samples offer ques. Hence, epitope retrieval methodologies are used the advantage of preserving proteins at the cellular and to recover the antigens; but recovery in some instances 1,4,5 subcellular levels and maintaining overall cell integrity in the can be less than optimal. An alternative to the use of tissue of interest without the use of chemical fixatives. To fixatives is the century-old method of freezing the tissue prevent specific or nonspecific degradation of proteins by at subzero temperatures. Freezing the tissue provides a autolytic and/or proteolytic processes, it is common practice snapshot of the cells as they would appear at the time the to immediately store frozen tissue sections obtained from a sample was removed from the organism, while avoiding cryostat under cryogenic conditions, for example  801C. Our degradation of intracellular molecules via autolysis or 6–8 laboratory recently challenged this widely http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied Immunohistochemistry & Molecular Morphology Wolters Kluwer Health

Snap-Frozen Brain Tissue Sections Stored With Desiccant at Ambient Laboratory Conditions Without Chemical Fixation are Resistant to Degradation for a Minimum of 6 Months

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References (19)

ISSN
1541-2016
DOI
10.1097/PAI.0b013e3181853001
pmid
19521279
Publisher site
See Article on Publisher Site

Abstract

TECHNICAL ARTICLE Snap-Frozen Brain Tissue Sections Stored With Desiccant at Ambient Laboratory Conditions Without Chemical Fixation are Resistant to Degradation for a Minimum of 6 Months Theodore R. Sadler, MS,*w Ani C. Khodavirdi, PhD,* David R. Hinton, MD,* and Daniel P. Holschneider, MDw zyJ them less compatible with current molecular techni- 1–3 Abstract: Cryosectioned tissues from snap-frozen samples offer ques. Hence, epitope retrieval methodologies are used the advantage of preserving proteins at the cellular and to recover the antigens; but recovery in some instances 1,4,5 subcellular levels and maintaining overall cell integrity in the can be less than optimal. An alternative to the use of tissue of interest without the use of chemical fixatives. To fixatives is the century-old method of freezing the tissue prevent specific or nonspecific degradation of proteins by at subzero temperatures. Freezing the tissue provides a autolytic and/or proteolytic processes, it is common practice snapshot of the cells as they would appear at the time the to immediately store frozen tissue sections obtained from a sample was removed from the organism, while avoiding cryostat under cryogenic conditions, for example  801C. Our degradation of intracellular molecules via autolysis or 6–8 laboratory recently challenged this widely

Journal

Applied Immunohistochemistry & Molecular Morphology – Wolters Kluwer Health

Published: Mar 1, 2009

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