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Semiquantitation of Axonal Injury in Traumatically Damaged Brains Using Color Deconvolution

Semiquantitation of Axonal Injury in Traumatically Damaged Brains Using Color Deconvolution Introduction:In traumatic brain injury biomechanics, macroscale biomechanical events need to be correlated with microscale neuropathologic changes and improved quantitation of microscopic axonal injury is an essential component of lesion evaluation.Objectives:To develop a novel technique for automatically identifying injured amyloid precursor protein immunopositive axons and aggregating these observations over a macroscopic brain dissection.Methods:A color deconvolution method was adapted into Matlab to identify clusters of pixels with colors typical of amyloid precursor protein positive tissue from large-scale brain dissection.Results:The methodology is demonstrated in the brain of a sheep subjected to a controlled cortical indentation.Conclusions:The technique will be of interest to pathologists and bioengineers seeking to quantitate brain injury over macroscales. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied Immunohistochemistry & Molecular Morphology Wolters Kluwer Health

Semiquantitation of Axonal Injury in Traumatically Damaged Brains Using Color Deconvolution

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Publisher
Wolters Kluwer Health
Copyright
Copyright © 2015 Wolters Kluwer Health, Inc. All rights reserved.
ISSN
1062-3345
eISSN
1533-4058
DOI
10.1097/PAI.0000000000000273
Publisher site
See Article on Publisher Site

Abstract

Introduction:In traumatic brain injury biomechanics, macroscale biomechanical events need to be correlated with microscale neuropathologic changes and improved quantitation of microscopic axonal injury is an essential component of lesion evaluation.Objectives:To develop a novel technique for automatically identifying injured amyloid precursor protein immunopositive axons and aggregating these observations over a macroscopic brain dissection.Methods:A color deconvolution method was adapted into Matlab to identify clusters of pixels with colors typical of amyloid precursor protein positive tissue from large-scale brain dissection.Results:The methodology is demonstrated in the brain of a sheep subjected to a controlled cortical indentation.Conclusions:The technique will be of interest to pathologists and bioengineers seeking to quantitate brain injury over macroscales.

Journal

Applied Immunohistochemistry & Molecular MorphologyWolters Kluwer Health

Published: Apr 1, 2017

References