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Secretory Phenotypes of Endothelial Cells in Culture Comparison of Aortic, Venous, Capillary, and Corneal Endothelium

Secretory Phenotypes of Endothelial Cells in Culture Comparison of Aortic, Venous,... Endothelial cells from different tissues display variations in morphology, intercellular Junctions, cell surface and growth properties, in production of basal lamina components, both In vivo and in vitro. We have investigated the spectra of extracellular proteins secreted by bovine endothelial cells cultured from large vessels, cornea, and capillaries. Aortic, venous, and corneal endothelial cells displayed highly similar patterns of protein synthesis as judged by analysis the culture medium; the major products were flbronectln, a glycoprotein similar or identical to platelet thrombospondln, and Type III procollagen. Ion-exchange chromatography, followed by peptide mapping, confirmed the presence of EC, a novel endothelial collagen previously described In bovine aortic endothelial cell cultures. Minor variations were found in the collagens of the cell layers: Type III, the predominant Interstitial collagen, was associated with the basement membrane Types IV and V and, in the case of corneal endothelium, with Type I. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Arteriosclerosis Wolters Kluwer Health

Secretory Phenotypes of Endothelial Cells in Culture Comparison of Aortic, Venous, Capillary, and Corneal Endothelium

Arteriosclerosis , Volume 1 (6) – Nov 1, 1981

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Copyright
© 1981 by American Heart Association, Inc.
ISSN
0276-5047

Abstract

Endothelial cells from different tissues display variations in morphology, intercellular Junctions, cell surface and growth properties, in production of basal lamina components, both In vivo and in vitro. We have investigated the spectra of extracellular proteins secreted by bovine endothelial cells cultured from large vessels, cornea, and capillaries. Aortic, venous, and corneal endothelial cells displayed highly similar patterns of protein synthesis as judged by analysis the culture medium; the major products were flbronectln, a glycoprotein similar or identical to platelet thrombospondln, and Type III procollagen. Ion-exchange chromatography, followed by peptide mapping, confirmed the presence of EC, a novel endothelial collagen previously described In bovine aortic endothelial cell cultures. Minor variations were found in the collagens of the cell layers: Type III, the predominant Interstitial collagen, was associated with the basement membrane Types IV and V and, in the case of corneal endothelium, with Type I.

Journal

ArteriosclerosisWolters Kluwer Health

Published: Nov 1, 1981

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