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Reproducible and Reliable Real-time PCR Assay to Measure Mature Form of miR-141

Reproducible and Reliable Real-time PCR Assay to Measure Mature Form of miR-141 RESEARCH ARTICLE Reproducible and Reliable Real-time PCR Assay to Measure Mature Form of miR-141 Seyed Hamid Aghaee-Bakhtiari,* Ehsan Arefian, PhD,w Masoud Soleimani, PhD,z Farshid Noorbakhsh, PhD,y Siamak Mirab Samiee, PhD,8 Pezhman Fard-Esfahani, PhD,z and Reza Mahdian, PhD* Key Words: mir-141, real time PCR, prostate cancer, ovarian Abstract: miR-141 is one of the miRNAs that has significant cancer, colon cancer expression variations in different human malignancies including (Appl Immunohistochem Mol Morphol 2015;00:000–000) prostate cancer, hepatocellular carcinoma, renal cell carcinoma, pancreatic cancer, gastric cancer, and ovarian cancer. Fur- thermore, various studies have designated miR-141 as a prog- nostic and diagnostic biomarker in different types of cancer. iRNAs are small endogenous noncoding RNAs that Thus, accurate and precise quantification of miR-141 is very mplay a key role in controlling gene expression essential for clinical diagnostics. In this regard, development of a through binding to their target sites on the 3 UTR of reproducible and reliable assay for miR-141 can be the first step mRNAs. Several studies showed the significant role of to standardize quantification of this valuable biomarker for miRNAs in a broad range of biological activities and in vitro diagnostics assays. Using stem-loop approach, we de- their regulatory functions in varieties http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied Immunohistochemistry & Molecular Morphology Wolters Kluwer Health

Reproducible and Reliable Real-time PCR Assay to Measure Mature Form of miR-141

Mahdian, Reza
Applied Immunohistochemistry & Molecular Morphology , Volume Publish Ahead of Print – Mar 1, 2015
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Copyright
Copyright © 2015 Wolters Kluwer Health, Inc. All rights reserved.
ISSN
1541-2016
DOI
10.1097/PAI.0000000000000169
pmid
25789530
Publisher site
See Article on Publisher Site

Abstract

RESEARCH ARTICLE Reproducible and Reliable Real-time PCR Assay to Measure Mature Form of miR-141 Seyed Hamid Aghaee-Bakhtiari,* Ehsan Arefian, PhD,w Masoud Soleimani, PhD,z Farshid Noorbakhsh, PhD,y Siamak Mirab Samiee, PhD,8 Pezhman Fard-Esfahani, PhD,z and Reza Mahdian, PhD* Key Words: mir-141, real time PCR, prostate cancer, ovarian Abstract: miR-141 is one of the miRNAs that has significant cancer, colon cancer expression variations in different human malignancies including (Appl Immunohistochem Mol Morphol 2015;00:000–000) prostate cancer, hepatocellular carcinoma, renal cell carcinoma, pancreatic cancer, gastric cancer, and ovarian cancer. Fur- thermore, various studies have designated miR-141 as a prog- nostic and diagnostic biomarker in different types of cancer. iRNAs are small endogenous noncoding RNAs that Thus, accurate and precise quantification of miR-141 is very mplay a key role in controlling gene expression essential for clinical diagnostics. In this regard, development of a through binding to their target sites on the 3 UTR of reproducible and reliable assay for miR-141 can be the first step mRNAs. Several studies showed the significant role of to standardize quantification of this valuable biomarker for miRNAs in a broad range of biological activities and in vitro diagnostics assays. Using stem-loop approach, we de- their regulatory functions in varieties

Journal

Applied Immunohistochemistry & Molecular Morphology – Wolters Kluwer Health

Published: Mar 1, 2015

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