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ORIGINAL ARTICLE Rapid Intraoperative Immunohistochemical Evaluation of Sentinel Lymph Nodes for Metastatic Breast Carcinoma Elizabeth I. Johnston, MD,* Robyn A. Beach, MD,* Sandra M. Waldrop, PhD,w Diane Lawson, MTL,* and Cynthia Cohen, MD* entinel lymph node (SLN) biopsy has become an Objective: Sentinel lymph node (SLN) biopsy is an integral part Sintegral component of the surgical management of of the surgical management of patients with breast cancer. patients with breast cancer. This procedure is a practical Rapid immunohistochemistry (RIHC) has the potential to and accurate method of predicting axillary lymph node increase detection of metastatic carcinoma at the time of frozen metastasis in patients with no clinical evidence of 1–3 section consultation. The authors assessed the accuracy and metastatic disease. The presence of axillary lymph turnaround time of a newly developed RIHC method for node metastases is an important prognostic factor and is pancytokeratin (RIHC-CK). often the determinant for the choice of adjuvant 2,4,5 therapy. However, a complete axillary lymph node Methods: Sixty-six SLNs from 32 patients with breast carcinoma dissection may lead to significant morbidity, such as were examined for metastasis using the Zymed Sentinel Lymph lymphedema, wound seroma formation, vascular injury, Node Rapid IHC Kit. Intraoperative frozen
Applied Immunohistochemistry & Molecular Morphology – Wolters Kluwer Health
Published: Mar 1, 2006
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