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Quantitative Infrared Spectroscopy of Formalin-fixed, Paraffin-embedded Tissue Specimens Paraffin Wax Removal With Organic Solvents

Quantitative Infrared Spectroscopy of Formalin-fixed, Paraffin-embedded Tissue Specimens Paraffin... TECHNICAL ARTICLE Quantitative Infrared Spectroscopy of Formalin-fixed, Paraffin-embedded Tissue Specimens Paraffin Wax Removal With Organic Solvents Curtis W. Meuse, PhD and Peter E. Barker, PhD classical preanalytical processing of medical diagnostic Abstract: Formalin-fixed, paraffin-embedded tissue specimens specimens has been challenging. The standard protocol form the basis for diagnostic histopathology. Although ade- for medical histopathology diagnosis often includes quate for morphologic visualization, clinical variability in incubation of tissue specimens in neutral-buffered for- preparation of formalin-fixed, paraffin-embedded clinical malin, followed by specimen dehydration and impregna- specimens represents an obstacle to quantitative molecular tion with liquid paraffin wax in nonpolar solvents. Tissue genetic analysis in areas such as genomics and proteomics. morphology in resulting paraffin-embedded tissue blocks A quantitative reexamination of classical histopathology tissue allows routine histomorphologic microscopic examina- preparation methods was initiated to determine which protocol tion by clinical pathologists. Nationwide, millions of steps might improve molecular analysis, beginning with patient-annotated, stored tissue blocks are archived. If deparaffinization. Infrared spectroscopy in the spectral region biologic and molecular information in such classically above 2000/cm of fixed sectioned model cell cultures through prepared medical specimens could be extracted in a glass microscope slides showed all solvents remove over 97% of meaningful and quantitative http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied Immunohistochemistry & Molecular Morphology Wolters Kluwer Health

Quantitative Infrared Spectroscopy of Formalin-fixed, Paraffin-embedded Tissue Specimens Paraffin Wax Removal With Organic Solvents

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References (18)

ISSN
1541-2016
DOI
10.1097/PAI.0b013e3181a9300e
pmid
19786865
Publisher site
See Article on Publisher Site

Abstract

TECHNICAL ARTICLE Quantitative Infrared Spectroscopy of Formalin-fixed, Paraffin-embedded Tissue Specimens Paraffin Wax Removal With Organic Solvents Curtis W. Meuse, PhD and Peter E. Barker, PhD classical preanalytical processing of medical diagnostic Abstract: Formalin-fixed, paraffin-embedded tissue specimens specimens has been challenging. The standard protocol form the basis for diagnostic histopathology. Although ade- for medical histopathology diagnosis often includes quate for morphologic visualization, clinical variability in incubation of tissue specimens in neutral-buffered for- preparation of formalin-fixed, paraffin-embedded clinical malin, followed by specimen dehydration and impregna- specimens represents an obstacle to quantitative molecular tion with liquid paraffin wax in nonpolar solvents. Tissue genetic analysis in areas such as genomics and proteomics. morphology in resulting paraffin-embedded tissue blocks A quantitative reexamination of classical histopathology tissue allows routine histomorphologic microscopic examina- preparation methods was initiated to determine which protocol tion by clinical pathologists. Nationwide, millions of steps might improve molecular analysis, beginning with patient-annotated, stored tissue blocks are archived. If deparaffinization. Infrared spectroscopy in the spectral region biologic and molecular information in such classically above 2000/cm of fixed sectioned model cell cultures through prepared medical specimens could be extracted in a glass microscope slides showed all solvents remove over 97% of meaningful and quantitative

Journal

Applied Immunohistochemistry & Molecular MorphologyWolters Kluwer Health

Published: Dec 1, 2009

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