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Optimization of Estrogen Receptor Analysis by Immunocytochemistry in Random Periareolar Fine-Needle Aspiration Samples of Breast Tissue Processed as Thin-Layer Preparations

Optimization of Estrogen Receptor Analysis by Immunocytochemistry in Random Periareolar... Immunostaining of estrogen receptor alpha (ER) in samples of benign breast tissue obtained by random periareolar fine-needle aspiration (RPFNA) is a practical tool for breast cancer chemoprevention trials. The authors report an optimized method of ER immunostaining for use with thin-layer preparations of modified Cytolyt-fixed benign breast tissue acquired by RPFNA. Samples of benign breast tissue and MCF-7 controls processed as thin-layer preparations were tested for the effects of antibody titer, antigen retrieval temperature (90° or 115°C), buffer (20% nuclear decloaker, pH 9.3; 10 mM citrate buffer, pH 6), and blocking solution (0.01% glucose oxidase or 0.3% H http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied Immunohistochemistry & Molecular Morphology Wolters Kluwer Health

Optimization of Estrogen Receptor Analysis by Immunocytochemistry in Random Periareolar Fine-Needle Aspiration Samples of Breast Tissue Processed as Thin-Layer Preparations

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ISSN
1062-3345
eISSN
1533-4058

Abstract

Immunostaining of estrogen receptor alpha (ER) in samples of benign breast tissue obtained by random periareolar fine-needle aspiration (RPFNA) is a practical tool for breast cancer chemoprevention trials. The authors report an optimized method of ER immunostaining for use with thin-layer preparations of modified Cytolyt-fixed benign breast tissue acquired by RPFNA. Samples of benign breast tissue and MCF-7 controls processed as thin-layer preparations were tested for the effects of antibody titer, antigen retrieval temperature (90° or 115°C), buffer (20% nuclear decloaker, pH 9.3; 10 mM citrate buffer, pH 6), and blocking solution (0.01% glucose oxidase or 0.3% H

Journal

Applied Immunohistochemistry & Molecular Morphology – Wolters Kluwer Health

Published: Sep 1, 2006

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