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New assays for monitoring residual HIV burden in effectively treated individuals

New assays for monitoring residual HIV burden in effectively treated individuals REVIEW URRENT New assays for monitoring residual HIV burden in PINION effectively treated individuals a,b a,b Matthew C. Strain and Douglas D. Richman Purpose of review Measurements of HIV burden have relied upon quantification of viral nucleic acids by real-time PCR (qPCR). To develop and test strategies for eradication, new methods are needed to better characterize residual cellular reservoirs in patients on suppressive antiretroviral therapy (ART). This review summarizes recent advances that may lead to clinically useful tests with improved sensitivity, reproducibility and throughput. Recent findings HIV DNA remains the most sensitive measure of residual infection, but its low levels are difficult to differentiate from assay noise by qPCR. Digital PCR has begun to improve the precision of existing real-time assays, but there remains a need to distinguish replication-competent proviruses. Rapid technological progress in single-cell analysis is beginning to offer new approaches, notably CyTOF and microengraving, which could provide vastly more information about the composition of the latent reservoir. Summary To investigate and assess therapies directed towards eradication, improved assays that simultaneously offer high sensitivity, precision and information content will be needed. Keywords cytometry via time of flight, digital PCR, droplet digital PCR, eradication, HIV DNA, HIV latency, microengraving, http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Current Opinion in HIV and Aids Wolters Kluwer Health

New assays for monitoring residual HIV burden in effectively treated individuals

Current Opinion in HIV and Aids , Volume 8 (2) – Mar 1, 2013

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Copyright
Copyright © Lippincott Williams & Wilkins. Unauthorized reproduction of this article is prohibited.
ISSN
1746-630X
eISSN
1746-6318
DOI
10.1097/COH.0b013e32835d811b
pmid
23314907
Publisher site
See Article on Publisher Site

Abstract

REVIEW URRENT New assays for monitoring residual HIV burden in PINION effectively treated individuals a,b a,b Matthew C. Strain and Douglas D. Richman Purpose of review Measurements of HIV burden have relied upon quantification of viral nucleic acids by real-time PCR (qPCR). To develop and test strategies for eradication, new methods are needed to better characterize residual cellular reservoirs in patients on suppressive antiretroviral therapy (ART). This review summarizes recent advances that may lead to clinically useful tests with improved sensitivity, reproducibility and throughput. Recent findings HIV DNA remains the most sensitive measure of residual infection, but its low levels are difficult to differentiate from assay noise by qPCR. Digital PCR has begun to improve the precision of existing real-time assays, but there remains a need to distinguish replication-competent proviruses. Rapid technological progress in single-cell analysis is beginning to offer new approaches, notably CyTOF and microengraving, which could provide vastly more information about the composition of the latent reservoir. Summary To investigate and assess therapies directed towards eradication, improved assays that simultaneously offer high sensitivity, precision and information content will be needed. Keywords cytometry via time of flight, digital PCR, droplet digital PCR, eradication, HIV DNA, HIV latency, microengraving,

Journal

Current Opinion in HIV and AidsWolters Kluwer Health

Published: Mar 1, 2013

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