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Multiplex Quantitative Measurement of mRNAs From Fixed Tissue Microarray Sections

Multiplex Quantitative Measurement of mRNAs From Fixed Tissue Microarray Sections REVIEW ARTICLE Multiplex Quantitative Measurement of mRNAs From Fixed Tissue Microarray Sections Michael Armani, PhD,*w Michael Tangrea, PhD,* Brian Yang, BS,* Alex Rosenberg, BA,* Kris Ylaya, BSc,z Jennifer Morris, BSc,z Jaime Rodriguez-Canales, MD,* Jeffrey Hanson, MSc,* Benjamin Shapiro, PhD,w Michael R. Emmert-Buck, MD, PhD,* Elisabeth Smela, PhD,y and Stephen M. Hewitt, MD, PhDz quently serve as the discovery method and are used to Abstract: The development of prognostic and diagnostic bio- measure a large number of genes in relatively few speci- markers, such as those from gene expression studies, requires mens. Subsequently, the candidate dysregulated genes independent validation in clinical specimens. Immuno- must be validated by measuring their expression in a histochemical analysis on tissue microarrays (TMAs) of for- larger independent sample set. At present, follow-up malin-fixed paraffin-embedded tissue is often used to increase validation of profiling data is relatively low throughput. the statistical power, and it is used more often than in situ hy- Therefore, it is common to perform immunohisto bridization, which can be technically limiting. Herein, we in- chemical analysis on a large cohort of patient samples of troduce a method for performing quantitative gene expression formalin-fixed and paraffin-embedded tissue (FFPE), in- analysis across a TMA http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied Immunohistochemistry & Molecular Morphology Wolters Kluwer Health

Multiplex Quantitative Measurement of mRNAs From Fixed Tissue Microarray Sections

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Copyright
Copyright © 2014 by Lippincott Williams & Wilkins
ISSN
1541-2016
eISSN
1533-4058
DOI
10.1097/PDM.0000000000000039
pmid
24809843
Publisher site
See Article on Publisher Site

Abstract

REVIEW ARTICLE Multiplex Quantitative Measurement of mRNAs From Fixed Tissue Microarray Sections Michael Armani, PhD,*w Michael Tangrea, PhD,* Brian Yang, BS,* Alex Rosenberg, BA,* Kris Ylaya, BSc,z Jennifer Morris, BSc,z Jaime Rodriguez-Canales, MD,* Jeffrey Hanson, MSc,* Benjamin Shapiro, PhD,w Michael R. Emmert-Buck, MD, PhD,* Elisabeth Smela, PhD,y and Stephen M. Hewitt, MD, PhDz quently serve as the discovery method and are used to Abstract: The development of prognostic and diagnostic bio- measure a large number of genes in relatively few speci- markers, such as those from gene expression studies, requires mens. Subsequently, the candidate dysregulated genes independent validation in clinical specimens. Immuno- must be validated by measuring their expression in a histochemical analysis on tissue microarrays (TMAs) of for- larger independent sample set. At present, follow-up malin-fixed paraffin-embedded tissue is often used to increase validation of profiling data is relatively low throughput. the statistical power, and it is used more often than in situ hy- Therefore, it is common to perform immunohisto bridization, which can be technically limiting. Herein, we in- chemical analysis on a large cohort of patient samples of troduce a method for performing quantitative gene expression formalin-fixed and paraffin-embedded tissue (FFPE), in- analysis across a TMA

Journal

Applied Immunohistochemistry & Molecular MorphologyWolters Kluwer Health

Published: May 1, 2014

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