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Microwave‐Stimulated Antigen Retrieval for Immunohistology and Immunoelectron Microscopy of Resin‐Embedded Sections

Microwave‐Stimulated Antigen Retrieval for Immunohistology and Immunoelectron Microscopy... This study describes a novel microwave (MW)-stimulated antigen-retrieval technique for LR White-embedded semithin and ultrathin sections. Six different types of antibodies [cytokeratin, vimentin, type IV collagen, β-catenin, immunoglobulin A (IgA) and IgG] were tested both with and without MW treatment. MW-treated sections revealed enhanced staining for cytokeratin, vimentin, type IV collagen, and β-catenin, but there was no distinguishable improvement in the immunolabeling of IgA and IgG. This method of antigen retrieval offers the possibility of combining immunohistology and ultrastructural immunolocalization with enhanced immunolabeling of selected antigens. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied Immunohistochemistry & Molecular Morphology Wolters Kluwer Health

Microwave‐Stimulated Antigen Retrieval for Immunohistology and Immunoelectron Microscopy of Resin‐Embedded Sections

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ISSN
1062-3345
eISSN
1533-4058

Abstract

This study describes a novel microwave (MW)-stimulated antigen-retrieval technique for LR White-embedded semithin and ultrathin sections. Six different types of antibodies [cytokeratin, vimentin, type IV collagen, β-catenin, immunoglobulin A (IgA) and IgG] were tested both with and without MW treatment. MW-treated sections revealed enhanced staining for cytokeratin, vimentin, type IV collagen, and β-catenin, but there was no distinguishable improvement in the immunolabeling of IgA and IgG. This method of antigen retrieval offers the possibility of combining immunohistology and ultrastructural immunolocalization with enhanced immunolabeling of selected antigens.

Journal

Applied Immunohistochemistry & Molecular MorphologyWolters Kluwer Health

Published: Jan 1, 1998

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