Microwave‐Stimulated Antigen Retrieval for Immunohistology and Immunoelectron Microscopy of Resin‐Embedded Sections
Microwave‐Stimulated Antigen Retrieval for Immunohistology and Immunoelectron Microscopy...
Sormunen, Raija ; -Y. Leong, Anthony S.
1998-01-01 00:00:00
This study describes a novel microwave (MW)-stimulated antigen-retrieval technique for LR White-embedded semithin and ultrathin sections. Six different types of antibodies [cytokeratin, vimentin, type IV collagen, β-catenin, immunoglobulin A (IgA) and IgG] were tested both with and without MW treatment. MW-treated sections revealed enhanced staining for cytokeratin, vimentin, type IV collagen, and β-catenin, but there was no distinguishable improvement in the immunolabeling of IgA and IgG. This method of antigen retrieval offers the possibility of combining immunohistology and ultrastructural immunolocalization with enhanced immunolabeling of selected antigens.
http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.pngApplied Immunohistochemistry & Molecular MorphologyWolters Kluwer Healthhttp://www.deepdyve.com/lp/wolters-kluwer-health/microwave-hyphen-stimulated-antigen-retrieval-for-immunohistology-and-Ui7kFpRdgZ
Microwave‐Stimulated Antigen Retrieval for Immunohistology and Immunoelectron Microscopy of Resin‐Embedded Sections
This study describes a novel microwave (MW)-stimulated antigen-retrieval technique for LR White-embedded semithin and ultrathin sections. Six different types of antibodies [cytokeratin, vimentin, type IV collagen, β-catenin, immunoglobulin A (IgA) and IgG] were tested both with and without MW treatment. MW-treated sections revealed enhanced staining for cytokeratin, vimentin, type IV collagen, and β-catenin, but there was no distinguishable improvement in the immunolabeling of IgA and IgG. This method of antigen retrieval offers the possibility of combining immunohistology and ultrastructural immunolocalization with enhanced immunolabeling of selected antigens.
Journal
Applied Immunohistochemistry & Molecular Morphology
– Wolters Kluwer Health
To get new article updates from a journal on your personalized homepage, please log in first, or sign up for a DeepDyve account if you don’t already have one.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.