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MIB-1 Cell Membrane Reactivity: A Finding That Should be Interpreted Carefully

MIB-1 Cell Membrane Reactivity: A Finding That Should be Interpreted Carefully LETTER TO THE EDITOR To the best of our knowledge, Therefore, we suggest that cell MIB-1 Cell Membrane the MIB-1 cell membrane and cyto- membrane positivity for MIB-1 could plasmic staining pattern have also be an artifact because of excessive Reactivity: A Finding been reported in pleomorphic adeno- heating of the tissue sections or, more That Should be ma of the salivary gland. Moreover generally, to thermal stress. Martin and Badra´ n observed a simi- On the other hand, this unusual Interpreted Carefully lar immunoreactivity also in tonsil staining for MIB-1 and the observa- crypt epithelial cells using a different tion of Tashiro et al, which described anti–Ki-67 monoclonal antibody the cell membrane expression of MIB- To the Editor: (Ki-88). 1 in 12 of 92 cases of salivary gland 1–6 We have read with interest the All authors believe that this pleomorphic adenoma, prompted us article by Leonardo et al in the June immunoreactivity for MIB-1 could be to analyze the MIB-1 reactivity in 18 2007 issue of Applied Immunohisto- because of a cytoplasmic and mem- cases of this tumor. In all cases, chemistry and Molecular Morphology branous antigen that cross-reacts with neoplastic cells did not show any regarding cell membrane reactivity of the MIB-1 antibody because it is membranous staining for MIB-1. antibodies to Ki-67 in human tumors. improbable that the cell cytoplasm In conclusion, our findings and The authors used 5 different anti–Ki-67 contains an epitope identical to the analysis of the results obtained by antibodies in different experimental nuclear proliferation antigen Ki-67. Leonardo et al in their article demon- conditions to verify the diagnostic role Recently, we have observed a strate that the cell membrane expression of membrane immunoreactivity in 6 consecutive series of 5 cases of infil- of MIB-1 is an artifact because it is not cases of thyroid hyalinizing trabecular trating ductal carcinoma of the possible to hypothesize a transmigra- adenoma, in 8 cases of pulmonary breast, stained for MIB-1 (1:100, tion of Ki-67 antigen from the nucleus sclerosing hemangiomas, and in 6 other DAKO), which showed, besides a to cytoplasm and cell membrane. tumors (4 invasive breast carcinomas, 1 nuclear staining, an unexpected cell Therefore, we think that the cell mem- sarcomatous pleural mesothelioma, membrane and cytoplasmic reactivity brane immunoreactivity for MIB-1 and 1 renal oncocytoma). They claim (Fig. 1). To explain this finding, should be interpreted carefully and used that only MIB-1 clone can be useful we checked the temperature of the with caution as a diagnostic clue. diagnostically in hyalinizing trabecular flattening bath for paraffin sections and found a value of 621C because adenoma of thyroid, as is well Rachele Del Sordo, MD 2,3 of temporary dysregulation of the known, but not in pulmonary scler- Angelo Sidoni, MD thermostat. Institute of Pathologic Anatomy and osing hemangioma, in contrast with Histology, Medical School, University of previous observations, and that mem- Subsequently, we have found Perugia, Italy brane immunoreactivity for MIB-1 can another case of invasive breast carci- be attributed to a cross-reactivity of the noma previously subjected to cryostat antibody with an epitope expressed at sectioning, showing a membranous REFERENCES thecellmembraneand relatedtoa and cytoplasmic immunoreactivity 1. Leonardo E, Volante M, Barbareschi M, et al. Cell membrane reactivity of MIB-1 peculiar neoplastic phenotype. for MIB-1. antibody to Ki67 in human tumors: fact or artifact? Appl Immunohistochem Mol Mor- phol. 2007;15:220–223. 2. Hirokawa M, Shimizu M, Manabe T, et al. Hyalinizing trabecular adenoma of the thyroid: its unusual cytoplasmic immunopo- sitivity for MIB1. Pathol Int. 1995;45: 399–401. 3. Hirokawa M, Carney JA. Cell membrane and cytoplasmic staining for MIB-1 in hyalinizing trabecular adenoma of the thyroid gland. Am J Surg Pathol. 2000;24: 575–578. 4. Hattori H. Sclerosing haemangioma of the lung is positive for MIB-1 in cell membrane and cytoplasmic staining pattern. Histo- pathology. 2002;40:291–293. 5. Tashiro T, Hirokawa M, Harada H, et al. Cell membrane expression of MIB-1 in salivary gland pleomorphic adenoma. His- topathology. 2002;41:559–561. 6. Martin CA, Badra´ n AF. Ki 67 reactivity in cell membrane and cytoplasm of tonsil crypt FIGURE 1. Strong membranous reactivity for MIB-1 in an infiltrating ductal epithelial cells. Appl Immunohistochem Mol carcinoma that underwent overheating during sectioning (200  ). Morphol. 2001;9:185–186. 568 Appl Immunohistochem Mol Morphol Volume 16, Number 6, December 2008 http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied Immunohistochemistry & Molecular Morphology Wolters Kluwer Health

MIB-1 Cell Membrane Reactivity: A Finding That Should be Interpreted Carefully

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ISSN
1541-2016
DOI
10.1097/PAI.0b013e31817af2cf
pmid
18800001
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Abstract

LETTER TO THE EDITOR To the best of our knowledge, Therefore, we suggest that cell MIB-1 Cell Membrane the MIB-1 cell membrane and cyto- membrane positivity for MIB-1 could plasmic staining pattern have also be an artifact because of excessive Reactivity: A Finding been reported in pleomorphic adeno- heating of the tissue sections or, more That Should be ma of the salivary gland. Moreover generally, to thermal stress. Martin and Badra´ n observed a simi- On the other hand, this unusual Interpreted Carefully lar immunoreactivity also in tonsil staining for MIB-1 and the observa- crypt epithelial cells using a different tion of Tashiro et al, which described anti–Ki-67 monoclonal antibody the cell membrane expression of MIB- To the Editor: (Ki-88). 1 in 12 of 92 cases of salivary gland 1–6 We have read with interest the All authors believe that this pleomorphic adenoma, prompted us article by Leonardo et al in the June immunoreactivity for MIB-1 could be to analyze the MIB-1 reactivity in 18 2007 issue of Applied Immunohisto- because of a cytoplasmic and mem- cases of this tumor. In all cases, chemistry and Molecular Morphology branous antigen that cross-reacts with neoplastic cells did not show any regarding cell membrane reactivity of the MIB-1 antibody because it is membranous staining for MIB-1. antibodies to Ki-67 in human tumors. improbable that the cell cytoplasm In conclusion, our findings and The authors used 5 different anti–Ki-67 contains an epitope identical to the analysis of the results obtained by antibodies in different experimental nuclear proliferation antigen Ki-67. Leonardo et al in their article demon- conditions to verify the diagnostic role Recently, we have observed a strate that the cell membrane expression of membrane immunoreactivity in 6 consecutive series of 5 cases of infil- of MIB-1 is an artifact because it is not cases of thyroid hyalinizing trabecular trating ductal carcinoma of the possible to hypothesize a transmigra- adenoma, in 8 cases of pulmonary breast, stained for MIB-1 (1:100, tion of Ki-67 antigen from the nucleus sclerosing hemangiomas, and in 6 other DAKO), which showed, besides a to cytoplasm and cell membrane. tumors (4 invasive breast carcinomas, 1 nuclear staining, an unexpected cell Therefore, we think that the cell mem- sarcomatous pleural mesothelioma, membrane and cytoplasmic reactivity brane immunoreactivity for MIB-1 and 1 renal oncocytoma). They claim (Fig. 1). To explain this finding, should be interpreted carefully and used that only MIB-1 clone can be useful we checked the temperature of the with caution as a diagnostic clue. diagnostically in hyalinizing trabecular flattening bath for paraffin sections and found a value of 621C because adenoma of thyroid, as is well Rachele Del Sordo, MD 2,3 of temporary dysregulation of the known, but not in pulmonary scler- Angelo Sidoni, MD thermostat. Institute of Pathologic Anatomy and osing hemangioma, in contrast with Histology, Medical School, University of previous observations, and that mem- Subsequently, we have found Perugia, Italy brane immunoreactivity for MIB-1 can another case of invasive breast carci- be attributed to a cross-reactivity of the noma previously subjected to cryostat antibody with an epitope expressed at sectioning, showing a membranous REFERENCES thecellmembraneand relatedtoa and cytoplasmic immunoreactivity 1. Leonardo E, Volante M, Barbareschi M, et al. Cell membrane reactivity of MIB-1 peculiar neoplastic phenotype. for MIB-1. antibody to Ki67 in human tumors: fact or artifact? Appl Immunohistochem Mol Mor- phol. 2007;15:220–223. 2. Hirokawa M, Shimizu M, Manabe T, et al. Hyalinizing trabecular adenoma of the thyroid: its unusual cytoplasmic immunopo- sitivity for MIB1. Pathol Int. 1995;45: 399–401. 3. Hirokawa M, Carney JA. Cell membrane and cytoplasmic staining for MIB-1 in hyalinizing trabecular adenoma of the thyroid gland. Am J Surg Pathol. 2000;24: 575–578. 4. Hattori H. Sclerosing haemangioma of the lung is positive for MIB-1 in cell membrane and cytoplasmic staining pattern. Histo- pathology. 2002;40:291–293. 5. Tashiro T, Hirokawa M, Harada H, et al. Cell membrane expression of MIB-1 in salivary gland pleomorphic adenoma. His- topathology. 2002;41:559–561. 6. Martin CA, Badra´ n AF. Ki 67 reactivity in cell membrane and cytoplasm of tonsil crypt FIGURE 1. Strong membranous reactivity for MIB-1 in an infiltrating ductal epithelial cells. Appl Immunohistochem Mol carcinoma that underwent overheating during sectioning (200  ). Morphol. 2001;9:185–186. 568 Appl Immunohistochem Mol Morphol Volume 16, Number 6, December 2008

Journal

Applied Immunohistochemistry & Molecular MorphologyWolters Kluwer Health

Published: Dec 1, 2008

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