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Lp(a) Phenotyping by Immunoblotting with Polyclonal and Monoclonal Antibodies

Lp(a) Phenotyping by Immunoblotting with Polyclonal and Monoclonal Antibodies A new method that allows rapid phenotyping of genetic Lp(a) glycoproteln types In large numbers of samples Is described. The method is based on sodium dodecyl sulfate gel electrophoresls of reduced serum or plasma In horizontal slab gels followed by Immunoblotting with polyclonal antl-Lp(a) llpoproteln or monoclonal anti- Lp(a) glycoproteln antibodies. Phenotyping of 194 unrelated, healthy subjects resulted In Lp(a) allele frequencies of Lp(a) http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Arteriosclerosis, Thrombosis, and Vascular Biology Wolters Kluwer Health

Lp(a) Phenotyping by Immunoblotting with Polyclonal and Monoclonal Antibodies

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Copyright
© 1988 by American Heart Association, Inc.
ISSN
1079-5642
eISSN
1524-4636

Abstract

A new method that allows rapid phenotyping of genetic Lp(a) glycoproteln types In large numbers of samples Is described. The method is based on sodium dodecyl sulfate gel electrophoresls of reduced serum or plasma In horizontal slab gels followed by Immunoblotting with polyclonal antl-Lp(a) llpoproteln or monoclonal anti- Lp(a) glycoproteln antibodies. Phenotyping of 194 unrelated, healthy subjects resulted In Lp(a) allele frequencies of Lp(a)

Journal

Arteriosclerosis, Thrombosis, and Vascular BiologyWolters Kluwer Health

Published: May 1, 1988

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