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Intact or Broken-apart RNA An Alternative Concept for ALK

Intact or Broken-apart RNA An Alternative Concept for ALK TECHNICAL ARTICLE Intact or Broken-apart RNA: An Alternative Concept for ALK Fusion Screening in Non–Small Cell Lung Cancer (NSCLC) Vassiliki Kotoula, MD, PhD,*w Mattheos Bobos, MD,w Maria Vassilakopoulou, MD,z Eleftheria Tsolaki, PhD,w Sofia Chrisafi, PhD,w Amanda Psyrri, MD,y George Lazaridis, MD,8 Kyriaki Papadopoulou, PhD,w Ioannis Efstratiou, MD,z Catherine Michail-Strantzia, MD,# Larisa V. Debelenko, MD, PhD,** Paris Kosmidis, MD,ww and George Fountzilas, MDw8 considered as originally misinterpreted by FISH in 3/121 cases Abstract: Anaplastic lymphoma kinase (ALK) break-apart flu- (2.5%). Tumors with >4 ALK gene copies were associated with orescent in situ hybridization (FISH) is currently used in diag- longer overall survival upon first-line chemotherapy. In con- nostics for the selection of non–small cell lung cancer (NSCLC) clusion, application of the ALK BAT test on routinely processed patients to receive crizotinib. We evaluated ALK status in NSCLC tissues yields the same fusion partner independent NSCLC with a novel ALK mRNA test based on the break-apart information as ALK break-apart FISH but is more robust FISH concept, which we called break-apart transcript (BAT) and cost-effective. The BAT concept may be considered for the 0 0 test. ALK5 and ALK3 transcript patterns were established with development of further drug-predictive translocation tests. qPCR http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied Immunohistochemistry & Molecular Morphology Wolters Kluwer Health

Intact or Broken-apart RNA An Alternative Concept for ALK

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Copyright
Copyright © 2014 by Lippincott Williams & Wilkins
ISSN
1541-2016
DOI
10.1097/PAI.0000000000000028
pmid
25153496
Publisher site
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Abstract

TECHNICAL ARTICLE Intact or Broken-apart RNA: An Alternative Concept for ALK Fusion Screening in Non–Small Cell Lung Cancer (NSCLC) Vassiliki Kotoula, MD, PhD,*w Mattheos Bobos, MD,w Maria Vassilakopoulou, MD,z Eleftheria Tsolaki, PhD,w Sofia Chrisafi, PhD,w Amanda Psyrri, MD,y George Lazaridis, MD,8 Kyriaki Papadopoulou, PhD,w Ioannis Efstratiou, MD,z Catherine Michail-Strantzia, MD,# Larisa V. Debelenko, MD, PhD,** Paris Kosmidis, MD,ww and George Fountzilas, MDw8 considered as originally misinterpreted by FISH in 3/121 cases Abstract: Anaplastic lymphoma kinase (ALK) break-apart flu- (2.5%). Tumors with >4 ALK gene copies were associated with orescent in situ hybridization (FISH) is currently used in diag- longer overall survival upon first-line chemotherapy. In con- nostics for the selection of non–small cell lung cancer (NSCLC) clusion, application of the ALK BAT test on routinely processed patients to receive crizotinib. We evaluated ALK status in NSCLC tissues yields the same fusion partner independent NSCLC with a novel ALK mRNA test based on the break-apart information as ALK break-apart FISH but is more robust FISH concept, which we called break-apart transcript (BAT) and cost-effective. The BAT concept may be considered for the 0 0 test. ALK5 and ALK3 transcript patterns were established with development of further drug-predictive translocation tests. qPCR

Journal

Applied Immunohistochemistry & Molecular Morphology – Wolters Kluwer Health

Published: Jan 1, 2015

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