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Increased Detection of Metastatic Melanoma in Pediatric Sentinel Lymph Node Biopsies Using RT-PCR on Paraffin-embedded Tissue

Increased Detection of Metastatic Melanoma in Pediatric Sentinel Lymph Node Biopsies Using RT-PCR... RESEARCH ARTICLE Increased Detection of Metastatic Melanoma in Pediatric Sentinel Lymph Node Biopsies Using RT-PCR on Paraffin-embedded Tissue Gino R. Somers, MBBS, PhD, FRCPA,*w Susan Chilton-MacNeill, BSc,* Keqin Zheng, PhD,* Mary Stuart, BScN, MN,z Alberto Pappo, MD,z Maria Zielenska, PhD,*w and Paul S. Thorner, MD, PhD*w provides a useful tool to determine the clinical significance of Abstract: Sentinel lymph node (SLN) biopsy has become RT-PCR positivity in melanoma SLNs. integral in the staging of patients with melanoma, and entails Key Words: pediatric, melanoma, sentinel lymph node biopsy, detailed histologic examination with immunohistochemistry. immunohistochemistry, RT-PCR, tyrosinase Reverse transcriptase-polymerase chain reaction (RT-PCR) for tyrosinase transcripts has been used to increase sensitivity but (Appl Immunohistochem Mol Morphol 2010;18:365–370) requires a dedicated piece of tissue that does not undergo histologic examination. We developed a nested RT-PCR assay for tyrosinase applicable on paraffin-embedded tissue and applied this to a series of SLNs from pediatric patients with entinel lymph node (SLN) biopsy has become an melanoma. Thirty-six SLNs from 4 females and 4 males were Sintegral part of the management of cutaneous malig- 1–3 nant melanoma. The presence of positive sentinel included in the study. Eight lymph nodes with reactive changes lymph nodes (SLNs) confers http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied Immunohistochemistry & Molecular Morphology Wolters Kluwer Health

Increased Detection of Metastatic Melanoma in Pediatric Sentinel Lymph Node Biopsies Using RT-PCR on Paraffin-embedded Tissue

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1541-2016

Abstract

RESEARCH ARTICLE Increased Detection of Metastatic Melanoma in Pediatric Sentinel Lymph Node Biopsies Using RT-PCR on Paraffin-embedded Tissue Gino R. Somers, MBBS, PhD, FRCPA,*w Susan Chilton-MacNeill, BSc,* Keqin Zheng, PhD,* Mary Stuart, BScN, MN,z Alberto Pappo, MD,z Maria Zielenska, PhD,*w and Paul S. Thorner, MD, PhD*w provides a useful tool to determine the clinical significance of Abstract: Sentinel lymph node (SLN) biopsy has become RT-PCR positivity in melanoma SLNs. integral in the staging of patients with melanoma, and entails Key Words: pediatric, melanoma, sentinel lymph node biopsy, detailed histologic examination with immunohistochemistry. immunohistochemistry, RT-PCR, tyrosinase Reverse transcriptase-polymerase chain reaction (RT-PCR) for tyrosinase transcripts has been used to increase sensitivity but (Appl Immunohistochem Mol Morphol 2010;18:365–370) requires a dedicated piece of tissue that does not undergo histologic examination. We developed a nested RT-PCR assay for tyrosinase applicable on paraffin-embedded tissue and applied this to a series of SLNs from pediatric patients with entinel lymph node (SLN) biopsy has become an melanoma. Thirty-six SLNs from 4 females and 4 males were Sintegral part of the management of cutaneous malig- 1–3 nant melanoma. The presence of positive sentinel included in the study. Eight lymph nodes with reactive changes lymph nodes (SLNs) confers

Journal

Applied Immunohistochemistry & Molecular Morphology – Wolters Kluwer Health

Published: Jul 1, 2010

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