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TECHNICAL ARTICLE Immunohistochemistry of Breast Tumor Markers on Archived Bouin-fixed Paraffin-embedded Tissues Jean-Franc¸ois Gagnon, MSc,*w z Franc¸ois Sanschagrin, PhD,*w zy Simon Jacob, MD,*w zy Louise Provencher, MD,w zy Carol Morin, PhD,*w zy and Caroline Diorio, PhDw zy rchived tumor specimens from patient with long-term 1,2 Abstract: Neutral-buffered formalin is the most commonly used Afollow-up are useful to find new clinical markers. tissue fixative in pathology laboratory. Among other fixatives, Neutral-buffered formalin is the most commonly used Bouin’s solution has been used in several laboratories and is still fixative in tissue pathology but historically, Bouin’s 3,4 in use for particular tissues. In this project, we determine if we solution was used as fixative in several laboratories. can study breast clinical markers on archived Bouin-fixed tissue Therefore, archived specimens fixed in Bouin’s solution, samples with immunohistochemistry (IHC) protocols optimized such as breast tumors, are common. To perform new for tissue fixed in neutral-buffered formalin. To evaluate the research projects, archived Bouin-fixed specimens have to concordance of IHC results between formalin-fixed and Bouin- be characterized with new markers, different antibody fixed tissues, we calculated the concordance percentage and clones, and different techniques. the k statistic of 12 clinical IHC markers quantified by
Applied Immunohistochemistry & Molecular Morphology – Wolters Kluwer Health
Published: Mar 1, 2011
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