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Immunohistochemical Staining of Papillary Breast Lesions

Immunohistochemical Staining of Papillary Breast Lesions RESEARCH ARTICLE Megan L. Troxell, MD, PhD, Marilyn Masek, BA, and Richard K. Sibley, MD 1–5 cells. Difficulty in interpretation of papillary lesions Abstract: The separation of ductal papilloma from intraductal of the breast often arises because both papilloma and papillary carcinoma of the breast on hematoxylin and eosin papillary carcinoma may consist of multiple layers of stained sections often presents diagnostic difficulty. Immuno- architecturally complex epithelial proliferations, in which histochemical staining is often employed in diagnosis, histori- myoepithelial cells are not readily discernible on hema- cally with smooth muscle actin (SMA). In this study, the toxylin and eosin (H&E) stained sections. Furthermore, staining characteristics of a panel of myoepithelial markers the biopsy sample may be small, or distorted by the (calponin, p63, P-cadherin), were compared with SMA, and the biopsy procedure, or by suboptimal fixation. In the past, epithelial expression of CD44s was assessed in 99 papillary the interpretation of these complex proliferative papillary lesions. SMA, calponin, and p63 demonstrated myoepithelial lesions often relied upon the presence of ductal carcinoma cells in 61%, 63%, and 65% of papillary lesions, respectively. in situ in adjacent terminal duct/lobular units or However, specificity was quite variable. Calponin-stained infiltrating ductal carcinoma, or http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied Immunohistochemistry & Molecular Morphology Wolters Kluwer Health

Immunohistochemical Staining of Papillary Breast Lesions

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ISSN
1541-2016
DOI
10.1097/01.pai.0000210420.45869.f4
pmid
17525625
Publisher site
See Article on Publisher Site

Abstract

RESEARCH ARTICLE Megan L. Troxell, MD, PhD, Marilyn Masek, BA, and Richard K. Sibley, MD 1–5 cells. Difficulty in interpretation of papillary lesions Abstract: The separation of ductal papilloma from intraductal of the breast often arises because both papilloma and papillary carcinoma of the breast on hematoxylin and eosin papillary carcinoma may consist of multiple layers of stained sections often presents diagnostic difficulty. Immuno- architecturally complex epithelial proliferations, in which histochemical staining is often employed in diagnosis, histori- myoepithelial cells are not readily discernible on hema- cally with smooth muscle actin (SMA). In this study, the toxylin and eosin (H&E) stained sections. Furthermore, staining characteristics of a panel of myoepithelial markers the biopsy sample may be small, or distorted by the (calponin, p63, P-cadherin), were compared with SMA, and the biopsy procedure, or by suboptimal fixation. In the past, epithelial expression of CD44s was assessed in 99 papillary the interpretation of these complex proliferative papillary lesions. SMA, calponin, and p63 demonstrated myoepithelial lesions often relied upon the presence of ductal carcinoma cells in 61%, 63%, and 65% of papillary lesions, respectively. in situ in adjacent terminal duct/lobular units or However, specificity was quite variable. Calponin-stained infiltrating ductal carcinoma, or

Journal

Applied Immunohistochemistry & Molecular MorphologyWolters Kluwer Health

Published: Jun 1, 2007

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