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Hybrid Capture-2 is Better Than In Situ Hybridization: A Study Comparison With Cervical Biopsies

Hybrid Capture-2 is Better Than In Situ Hybridization: A Study Comparison With Cervical... LETTERS TO THE EDITOR Hybrid Capture-2 AB is Better Than In Situ Hybridization: A Study Comparison With Cervical Biopsies To the Editor: Afify et al recently reported the successful use of in situ hybridization (ISH) for human papillomavirus (HPV) diagnosis on cell blocks prepared from FIGURE 1. ISH-positive staining for HPV. Ventana ISH staining at  30 for HPV. residual SurePath (TriPath Imaging, A, Positive stain with diffuse pattern is noted at the arrow. B, Positive stain with Burlington, VT) Pap material. They punctate pattern (see arrow). ISH indicates in situ hybridization; HPV, human conclude that ISH and immunohisto- papillomavirus. chemistry may be the most practical choice for routine HPV screening and raise concerns about false negatives part of Roche Diagnostics). They were Probe cocktails for the 2 methods differ with the hybrid capture-2 (HC-2) assay evaluated for accuracy with respect to in that the ISH cocktail contains probe (QIAGEN, Inc, Valencia, CA). Their true presence or absence of disease to HPV 70, a probe not found in the results were not compared directly to using available biopsy and cytology HC-2 reagent, and the HC-2 cocktail HC-2, although it was noted that this is data from patient charts. Both http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied Immunohistochemistry & Molecular Morphology Wolters Kluwer Health

Hybrid Capture-2 is Better Than In Situ Hybridization: A Study Comparison With Cervical Biopsies

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ISSN
1541-2016
DOI
10.1097/PAI.0b013e3181bb5022
pmid
19809301
Publisher site
See Article on Publisher Site

Abstract

LETTERS TO THE EDITOR Hybrid Capture-2 AB is Better Than In Situ Hybridization: A Study Comparison With Cervical Biopsies To the Editor: Afify et al recently reported the successful use of in situ hybridization (ISH) for human papillomavirus (HPV) diagnosis on cell blocks prepared from FIGURE 1. ISH-positive staining for HPV. Ventana ISH staining at  30 for HPV. residual SurePath (TriPath Imaging, A, Positive stain with diffuse pattern is noted at the arrow. B, Positive stain with Burlington, VT) Pap material. They punctate pattern (see arrow). ISH indicates in situ hybridization; HPV, human conclude that ISH and immunohisto- papillomavirus. chemistry may be the most practical choice for routine HPV screening and raise concerns about false negatives part of Roche Diagnostics). They were Probe cocktails for the 2 methods differ with the hybrid capture-2 (HC-2) assay evaluated for accuracy with respect to in that the ISH cocktail contains probe (QIAGEN, Inc, Valencia, CA). Their true presence or absence of disease to HPV 70, a probe not found in the results were not compared directly to using available biopsy and cytology HC-2 reagent, and the HC-2 cocktail HC-2, although it was noted that this is data from patient charts. Both

Journal

Applied Immunohistochemistry & Molecular Morphology – Wolters Kluwer Health

Published: Mar 1, 2010

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