Access the full text.
Sign up today, get DeepDyve free for 14 days.
Y. Jo, M. Choi, S. Song, M. Kim, N. Yoo, S. Lee (2016)
Frameshift Mutations of HSPA4 and MED13 in Gastric and Colorectal CancersPathology & Oncology Research, 22
Eun Choi, M. Kim, S. Song, N. Yoo, S. Lee (2016)
Intratumoral Heterogeneity of Frameshift Mutations in MECOM Gene is Frequent in Colorectal Cancers with High Microsatellite InstabilityPathology & Oncology Research, 23
M. Lawrence, P. Stojanov, C. Mermel, L. Garraway, T. Golub, M. Meyerson, S. Gabriel, E. Lander, G. Getz (2014)
Discovery and saturation analysis of cancer genes across 21 tumor typesNature, 505
Hui Ye, Tianwei Yu, Stephane Temam, Barry Ziober, Jianguang Wang, Joel Schwartz, Li Mao, David Wong, Xiaofeng Zhou (2008)
Transcriptomic dissection of tongue squamous cell carcinomaBMC Genomics, 9
N. Mcgranahan, C. Swanton (2015)
Biological and therapeutic impact of intratumor heterogeneity in cancer evolution.Cancer cell, 27 1
Tae-Min Kim, Seung-Hyun Jung, In-Pyo Baek, Sung‐Hak Lee, Y. Choi, Ji‐Youl Lee, Y. Chung, S. Lee (2014)
Regional biases in mutation screening due to intratumoural heterogeneity of prostate cancerThe Journal of Pathology, 233
V. Almendro, Yu-Kang Cheng, A. Randles, S. Itzkovitz, A. Marusyk, E. Ametller, X. Gonzalez-Farre, M. Muñoz, H. Russnes, Å. Helland, I. Rye, A. Børresen-Dale, R. Maruyama, A. Oudenaarden, M. Dowsett, Robin Jones, J. Reis-Filho, P. Gascón, M. Gönen, F. Michor, K. Polyak (2014)
Inference of tumor evolution during chemotherapy by computational modeling and in situ analysis of genetic and phenotypic cellular diversity.Cell reports, 6 3
K. Imai, Hiroyuki Yamamoto (2008)
Carcinogenesis and microsatellite instability: the interrelationship between genetics and epigenetics.Carcinogenesis, 29 4
Yang Yu, Vijay Walia, R. Elble (2013)
Loss of CLCA4 Promotes Epithelial-to-Mesenchymal Transition in Breast Cancer CellsPLoS ONE, 8
Bo Yang, Lin Cao, B. Liu, C. McCaig, Jin Pu (2013)
The Transition from Proliferation to Differentiation in Colorectal Cancer Is Regulated by the Calcium Activated Chloride Channel A1PLoS ONE, 8
A. Gruber, B. Pauli (1999)
Tumorigenicity of human breast cancer is associated with loss of the Ca2+-activated chloride channel CLCA2.Cancer research, 59 21
Ju Lee, Y. Choi, E. Je, HO Kim, N. Yoo, S. Lee (2016)
Frameshift mutation of WISP3 gene and its regional heterogeneity in gastric and colorectal cancers.Human pathology, 50
A. Marusyk, V. Almendro, K. Polyak (2012)
Intra-tumour heterogeneity: a looking glass for cancer?Nature Reviews Cancer, 12
G. Calin, R. Gafà, M. Tibiletti, V. Herlea, G. Becheanu, L. Cavazzini, G. Barbanti‐Brodano, I. Nenci, M. Negrini, G. Lanza (2000)
Genetic progression in microsatellite instability high (MSI‐H) colon cancers correlates with clinico‐pathological parameters: A study of the TGRβRII, BAX, hMSH3, hMSH6, IGFIIR and BLM genesInternational Journal of Cancer, 89
Xiangzhao Li, Huanjiao Liu, Minyi Liang, Huihui Chen, Li Liang (2020)
[Clinicopathological features and types of microsatellite instability in 1394 patients with colorectal cancer].Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 40 11
A. Gonzalez-Perez, Christian Perez-Llamas, Jordi Deu-Pons, D. Tamborero, Michael Schroeder, Alba Jené-Sanz, Alberto Santos, N. López-Bigas (2013)
IntOGen-mutations identifies cancer drivers across tumor typesNature Methods, 10
K. Pawłowski, M. Lepistö, N. Meinander, U. Sivars, Mikael Varga, E. Wieslander (2006)
Novel conserved hydrolase domain in the CLCA family of alleged calcium‐activated chloride channelsProteins: Structure, 63
M. Loewen, G. Forsyth (2005)
Structure and function of CLCA proteins.Physiological reviews, 85 3
Murphy (2006)
Comparison of the microsatellite instability analysis system and the Bethesda panel for the determination of microsatellite instability in colorectal cancersJ Mol Diagn, 8
M. Agnel, T. Vermat, J. Culouscou (1999)
Identification of three novel members of the calcium‐dependent chloride channel (CaCC) family predominantly expressed in the digestive tract and trachea 1FEBS Letters, 455
B. Pauli, M. Abdel-Ghany, Hung-chi Cheng, Achim Gruber, H. Archibald, R. Elble (2000)
Molecular Characteristics And Functional Diversity Of Clca Family MembersClinical and Experimental Pharmacology and Physiology, 27
Chloride channel calcium-activated (CLCA) genes encode regulators for chloride transport across the cell membrane. As for cancer development, some CLCA genes are considered putative tumor suppressor genes. The aim of this study was to explore whether CLCA4 gene would have mutations in its nucleotide repeats in colorectal cancer (CRC). In a public database, we found that CLCA4 gene had mononucleotide repeats in the coding sequences that might be mutational targets in the cancers with microsatellite instability. For this, the current study studied 146 CRCs for mutation and expression analyses by single-strand conformation polymorphism analysis, DNA sequencing, and immunohistochemistry. Overall, we found CLCA4 frameshift mutations in 12/101 (11.8%) CRCs with high-microsatellite instability (MSI-H), but none in microsatellite stable CRCs (0/45) (P<0.01). In addition, we analyzed intratumoral heterogeneity of the CLCA4 frameshift mutations and found that 1 CRC harbored regional intratumoral heterogeneity of the CLCA4 frameshift mutation. Loss of CLCA4 protein expression was identified in 50% of CRCs. Also, cancers with MSI-H harboring CLCA4 frameshift mutations showed lower CLCA4 immunostaining than those with the wild-type. Our data indicate that the CLCA4 gene harbors alterations both in somatic mutation and expression, suggesting their roles in tumorigenesis of CRC with MSI-H.
Applied Immunohistochemistry & Molecular Morphology – Wolters Kluwer Health
Published: Aug 6, 2020
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.