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Experimental Validation of Peptide Immunohistochemistry Controls

Experimental Validation of Peptide Immunohistochemistry Controls Downloaded from http://journals.lww.com/appliedimmunohist by BhDMf5ePHKbH4TTImqenVA5KvPVPZ0P5BEgU+IUTEfzO/GUWifn2IfwcEVVH9SSn on 06/10/2020 TECHNICAL ARTICLE Experimental Validation of Peptide Immunohistochemistry Controls Steven A. Bogen, MD, PhD,*w Kodela Vani, MS,* Brian McGraw, BSME,z Vin Federico, BSEE,z Iqbal Habib, HBSc, MBA,y Ron Zeheb, PhD,J Ed Luther, AB,z Colin Tristram, MSc,# and Seshi R. Sompuram, PhD*w (Appl Immunohistochem Mol Morphol 2009;17:239–246) Abstract: Peptide immunohistochemistry (IHC) controls are a new quality control format for verifying proper IHC assay performance, offering advantages in high throughput automated ith the widespread use of immunohistochemical manufacture and standardization. We previously demonstrated Wmethods for producing semiquantitative data, there that formalin-fixed peptide epitopes, covalently attached to glass is broad agreement that more rigorous quality assurance microscope slides, behaved (immunochemically) in a similar methods are required. Although most attention has focused fashion to the native protein in tissue sections. To convert this 1–4 on the accurate measurement of HER-2, similar needs promising idea into a practical clinical laboratory quality apply to other immunohistochemical markers, such as control tool, we tested the hypothesis that the quality assurance 5–8 estrogen and progesterone receptors, and epidermal information provided by peptide IHC controls accurately 9–11 growth factor receptor. The use of immunohistochem- reflects IHC staining performance among a diverse group http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied Immunohistochemistry & Molecular Morphology Wolters Kluwer Health

Experimental Validation of Peptide Immunohistochemistry Controls

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Publisher
Wolters Kluwer Health
ISSN
1541-2016
DOI
10.1097/PAI.0b013e3181904379
Publisher site
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Abstract

Downloaded from http://journals.lww.com/appliedimmunohist by BhDMf5ePHKbH4TTImqenVA5KvPVPZ0P5BEgU+IUTEfzO/GUWifn2IfwcEVVH9SSn on 06/10/2020 TECHNICAL ARTICLE Experimental Validation of Peptide Immunohistochemistry Controls Steven A. Bogen, MD, PhD,*w Kodela Vani, MS,* Brian McGraw, BSME,z Vin Federico, BSEE,z Iqbal Habib, HBSc, MBA,y Ron Zeheb, PhD,J Ed Luther, AB,z Colin Tristram, MSc,# and Seshi R. Sompuram, PhD*w (Appl Immunohistochem Mol Morphol 2009;17:239–246) Abstract: Peptide immunohistochemistry (IHC) controls are a new quality control format for verifying proper IHC assay performance, offering advantages in high throughput automated ith the widespread use of immunohistochemical manufacture and standardization. We previously demonstrated Wmethods for producing semiquantitative data, there that formalin-fixed peptide epitopes, covalently attached to glass is broad agreement that more rigorous quality assurance microscope slides, behaved (immunochemically) in a similar methods are required. Although most attention has focused fashion to the native protein in tissue sections. To convert this 1–4 on the accurate measurement of HER-2, similar needs promising idea into a practical clinical laboratory quality apply to other immunohistochemical markers, such as control tool, we tested the hypothesis that the quality assurance 5–8 estrogen and progesterone receptors, and epidermal information provided by peptide IHC controls accurately 9–11 growth factor receptor. The use of immunohistochem- reflects IHC staining performance among a diverse group

Journal

Applied Immunohistochemistry & Molecular Morphology – Wolters Kluwer Health

Published: May 1, 2009

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