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Original Articles Evaluation of a Novel F-Labeled Positron-Emission Tomography Perfusion Tracer for the Assessment of Myocardial Infarct Size in Rats Hossam M. Sherif, MD; Antti Saraste, MD, PhD; Eliane Weidl, PhD; Axel W. Weber, MS; Takahiro Higuchi, MD, PhD; Sybille Reder, BSc; Thorsten Poethko, PhD; Gjermund Henriksen, PhD; David Casebier, PhD; Simon Robinson, PhD; Hans-Ju¨rgen Wester, PhD; Stephan G. Nekolla, PhD; Markus Schwaiger, MD Background—The goal of this study was to evaluate a new F-labeled positron-emission tomography (PET) perfusion tracer, F BMS747158-02, for the assessment of myocardial infarct (MI) size. Methods and Results—Wistar rats were studied 24 hours after ligation of the left coronary artery either permanently (n15) or transiently (n16) for 30 minutes. Seven nonoperated rats were studied as controls. The rats were injected with 37 MBq of F BMS747158-02 and imaged with a small animal PET scanner for 20 minutes. Polar maps were generated for measurement of PET defect size, and left ventricular systolic and diastolic volumes were assessed in gated images. As a reference, MI size was determined by 2,3,5-triphenyltetrazolium chloride staining of left ventricular tissue samples. Permanent or transient ligation of the left coronary artery produced transmural or subendocardial MI of variable sizes, respectively. In
Circulation: Cardiovascular Imaging – Wolters Kluwer Health
Published: Mar 1, 2009
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