Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Enhanced Metabolism of Normolipidemic Human Plasma Very Low Density Lipoprotein in Cultured Cells by Exogenous Apolipoprotein E‐3

Enhanced Metabolism of Normolipidemic Human Plasma Very Low Density Lipoprotein in Cultured Cells... In this Investigation In cultured human fibroblasts, an attempt was made to determine the optimal metabolism of apolipoprotein (apo) B-100 llpoprotelns from normolipidemic human subjects. We supplemented culture systems containing 1KHlpoprotelns wtth exogenous recomblnant or plasmatlc apo E-3. Very low density lipoprotein (VLDL) fractions I, II, and III, and low density llpoprotelns (LDL) were prepared from one E 4/3 and four E 3/3 subjects. Without added apo E-3, cellular metabolism (binding, cell association, and degradation) of VLOL-I, II, and III was negligible. Exogenous apo E-3 caused a many-fold enhancement of the metabolism of the three VLDL fractions, but LDL was not affected. The effects of apo E-3 were specific, not observed with apo E-2, and not observed on receptor-negative cells. Exogenous apo E-3 also enhanced down-regulation of cellular sterol synthesis by the VLDLs, but not LDL, Indicating Increased particle catabotism by the cells. The optimal concentrations of exogenous apo E-3 were 4 to 6 /tg protein/15 /tg VLDL- protein, when most of the added apo E-3 became associated with the VLDL particles. Apo E-3 failed to associate with LDL. These results demonstrate that availability and association of adequate amounts of apo E-3 are crucial for optimal cellular metabolism of apo B-100 llpoprotelns along the VLDL→LDL cascade. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Arteriosclerosis Wolters Kluwer Health

Enhanced Metabolism of Normolipidemic Human Plasma Very Low Density Lipoprotein in Cultured Cells by Exogenous Apolipoprotein E‐3

Download PDF
8 pages

Loading next page...
 
/lp/wolters-kluwer-health/enhanced-metabolism-of-normolipidemic-human-plasma-very-low-density-uElH10Q3hE

References

References for this paper are not available at this time. We will be adding them shortly, thank you for your patience.

Copyright
© 1988 by American Heart Association, Inc.
ISSN
0276-5047

Abstract

In this Investigation In cultured human fibroblasts, an attempt was made to determine the optimal metabolism of apolipoprotein (apo) B-100 llpoprotelns from normolipidemic human subjects. We supplemented culture systems containing 1KHlpoprotelns wtth exogenous recomblnant or plasmatlc apo E-3. Very low density lipoprotein (VLDL) fractions I, II, and III, and low density llpoprotelns (LDL) were prepared from one E 4/3 and four E 3/3 subjects. Without added apo E-3, cellular metabolism (binding, cell association, and degradation) of VLOL-I, II, and III was negligible. Exogenous apo E-3 caused a many-fold enhancement of the metabolism of the three VLDL fractions, but LDL was not affected. The effects of apo E-3 were specific, not observed with apo E-2, and not observed on receptor-negative cells. Exogenous apo E-3 also enhanced down-regulation of cellular sterol synthesis by the VLDLs, but not LDL, Indicating Increased particle catabotism by the cells. The optimal concentrations of exogenous apo E-3 were 4 to 6 /tg protein/15 /tg VLDL- protein, when most of the added apo E-3 became associated with the VLDL particles. Apo E-3 failed to associate with LDL. These results demonstrate that availability and association of adequate amounts of apo E-3 are crucial for optimal cellular metabolism of apo B-100 llpoprotelns along the VLDL→LDL cascade.

Journal

Arteriosclerosis – Wolters Kluwer Health

Published: Sep 1, 1988

There are no references for this article.