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Effect of Storage on p63 Immunohistochemistry A Time-course Study

Effect of Storage on p63 Immunohistochemistry A Time-course Study RESEARCH ARTICLE Effect of Storage on p63 Immunohistochemistry A Time-course Study Holly N. Burford, MD, Amy L. Adams, MD, and Omar Hameed, MBChB 4–6 difficult prostate needle biopsies. Immunostains for p63 Abstract: One of the problems with immunohistochemistry is that have also been used in the evaluation of myoepithelial storage of unstained slides can have a detectable negative effect on differentiation in studies of basal-type breast carcino- 7,8 the intensity of staining for various antigens. This has not been ma, to identify epithelial/myoepithelial differentiation well studied for the p63 antigen. A time-course study was in metaplastic and sarcomatoid carcinomas of the 9,10 11 performed to evaluate the effect of storage on the intensity of breast and elsewhere, and also in the evaluation of 12 13 p63 immunohistochemical staining. Slides from various breast tumors of the skin, salivary glands, and uterine lesions/foci were stained with a p63 antibody on day 0 and after cervix among other locations. storage at room temperature for 2, 4, and 8 weeks. A p63 intensity One issue with p63 immunohistochemistry has been score, ranging from 0 to 300, was calculated using the intensity the recent finding of decreased intensity of staining in (0, 1, 2, http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied Immunohistochemistry & Molecular Morphology Wolters Kluwer Health

Effect of Storage on p63 Immunohistochemistry A Time-course Study

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ISSN
1541-2016
DOI
10.1097/PAI.0b013e31818110de
pmid
18815566
Publisher site
See Article on Publisher Site

Abstract

RESEARCH ARTICLE Effect of Storage on p63 Immunohistochemistry A Time-course Study Holly N. Burford, MD, Amy L. Adams, MD, and Omar Hameed, MBChB 4–6 difficult prostate needle biopsies. Immunostains for p63 Abstract: One of the problems with immunohistochemistry is that have also been used in the evaluation of myoepithelial storage of unstained slides can have a detectable negative effect on differentiation in studies of basal-type breast carcino- 7,8 the intensity of staining for various antigens. This has not been ma, to identify epithelial/myoepithelial differentiation well studied for the p63 antigen. A time-course study was in metaplastic and sarcomatoid carcinomas of the 9,10 11 performed to evaluate the effect of storage on the intensity of breast and elsewhere, and also in the evaluation of 12 13 p63 immunohistochemical staining. Slides from various breast tumors of the skin, salivary glands, and uterine lesions/foci were stained with a p63 antibody on day 0 and after cervix among other locations. storage at room temperature for 2, 4, and 8 weeks. A p63 intensity One issue with p63 immunohistochemistry has been score, ranging from 0 to 300, was calculated using the intensity the recent finding of decreased intensity of staining in (0, 1, 2,

Journal

Applied Immunohistochemistry & Molecular MorphologyWolters Kluwer Health

Published: Jan 1, 2009

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