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Double-Label Confocal Laser-Scanning Microscopy, Image Restoration, and Real-Time Three-Dimensional Reconstruction to Study Axons in the Central Nervous System and Their Contacts With Target Neurons

Double-Label Confocal Laser-Scanning Microscopy, Image Restoration, and Real-Time... The current double tracing–double confocal laser-scanning method was developed to reconstruct identified nerve fibers and their contacts with identified target neurons in the rat brain in three dimensions. It intends to fill the gap between conventional light microscopic and electron microscopic neuroanatomic tracing. The steps involved are as follows: (1) injection of two neuroanatomic tracers— http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied Immunohistochemistry & Molecular Morphology Wolters Kluwer Health

Double-Label Confocal Laser-Scanning Microscopy, Image Restoration, and Real-Time Three-Dimensional Reconstruction to Study Axons in the Central Nervous System and Their Contacts With Target Neurons

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ISSN
1062-3345
eISSN
1533-4058

Abstract

The current double tracing–double confocal laser-scanning method was developed to reconstruct identified nerve fibers and their contacts with identified target neurons in the rat brain in three dimensions. It intends to fill the gap between conventional light microscopic and electron microscopic neuroanatomic tracing. The steps involved are as follows: (1) injection of two neuroanatomic tracers—

Journal

Applied Immunohistochemistry & Molecular MorphologyWolters Kluwer Health

Published: Mar 1, 2002

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