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Determination of Optimum Formalin Fixation Duration for Prostate Needle Biopsies for Immunohistochemistry and Quantum Dot FISH Analysis

Determination of Optimum Formalin Fixation Duration for Prostate Needle Biopsies for... RESEARCH ARTICLE Determination of Optimum Formalin Fixation Duration for Prostate Needle Biopsies for Immunohistochemistry and Quantum Dot FISH Analysis Ubaradka G. Sathyanarayana, PhD,* Chandler Birch, BSc,* Raymond B. Nagle, MD, PhD,w Scott A. Tomlins, MD, PhD,zy8 Nallasivam Palanisamy, PhD,z8 Wenjun Zhang, PhD,* Antony Hubbard, BSc,* Patrick Brunhoeber, MD,* Yixin Wang, PhD,* and Lei Tang, PhD* cohort of 18 routinely processed prostate biopsy cores, all cores Abstract: Prostate biopsy is the key clinical specimen for disease were stained successfully with the QD ISH assay, and results diagnosis. However, various conditions used during biopsy were 100% concordant to ERG and PTEN IHC. We conclude processing for histologic analysis may affect the performance of that 8 to 72 hours duration of fixation for prostate needle diagnostic tests, such as hematoxylin and eosin (H&E) staining, biopsies in 10% NBF results in optimal QD ISH assay immunohistochemistry (IHC), or in situ hybridization (ISH). performance. One such condition that may affect diagnostic test performance Key Words: prostate cancer, gene expression, formalin fixed is fixation duration in 10% neutral buffered formalin (NBF). paraffin embedded, IHC, quantum dot, tissue diagnostic For example, prostate needle biopsies are often <1 mm in dia- meter and thus overfixed. It is http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied Immunohistochemistry & Molecular Morphology Wolters Kluwer Health

Determination of Optimum Formalin Fixation Duration for Prostate Needle Biopsies for Immunohistochemistry and Quantum Dot FISH Analysis

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References (30)

Copyright
Copyright © 2014 Wolters Kluwer Health, Inc. All rights reserved.
ISSN
1541-2016
DOI
10.1097/PAI.0000000000000085
pmid
25265431
Publisher site
See Article on Publisher Site

Abstract

RESEARCH ARTICLE Determination of Optimum Formalin Fixation Duration for Prostate Needle Biopsies for Immunohistochemistry and Quantum Dot FISH Analysis Ubaradka G. Sathyanarayana, PhD,* Chandler Birch, BSc,* Raymond B. Nagle, MD, PhD,w Scott A. Tomlins, MD, PhD,zy8 Nallasivam Palanisamy, PhD,z8 Wenjun Zhang, PhD,* Antony Hubbard, BSc,* Patrick Brunhoeber, MD,* Yixin Wang, PhD,* and Lei Tang, PhD* cohort of 18 routinely processed prostate biopsy cores, all cores Abstract: Prostate biopsy is the key clinical specimen for disease were stained successfully with the QD ISH assay, and results diagnosis. However, various conditions used during biopsy were 100% concordant to ERG and PTEN IHC. We conclude processing for histologic analysis may affect the performance of that 8 to 72 hours duration of fixation for prostate needle diagnostic tests, such as hematoxylin and eosin (H&E) staining, biopsies in 10% NBF results in optimal QD ISH assay immunohistochemistry (IHC), or in situ hybridization (ISH). performance. One such condition that may affect diagnostic test performance Key Words: prostate cancer, gene expression, formalin fixed is fixation duration in 10% neutral buffered formalin (NBF). paraffin embedded, IHC, quantum dot, tissue diagnostic For example, prostate needle biopsies are often <1 mm in dia- meter and thus overfixed. It is

Journal

Applied Immunohistochemistry & Molecular MorphologyWolters Kluwer Health

Published: May 1, 2015

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