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Correlation of IHC and FISH for ALK IHC Score Algorithm for FISH

Correlation of IHC and FISH for ALK IHC Score Algorithm for FISH ORIGINAL ARTICLE Correlation of IHC and FISH for ALK Gene Rearrangement in Non-small Cell Lung Carcinoma IHC Score Algorithm for FISH Eunhee S. Yi, MD,* Jennifer M. Boland, MD,* Joseph J. Maleszewski, MD,* Anja C. Roden, MD,* Andre M. Oliveira, MD,* Marie-Christine Aubry, MD,* Michele R. Erickson-Johnson, MS,* Bolette L. Caron, BA,* Yan Li, MD,† Hui Tang, PhD,‡ Shawn Stoddard, RN,† Jason Wampfler, BS,‡ Kimary Kulig, PhD, MPH,§ and Ping Yang, MD, PhD† ALK1 monoclonal antibody with ADVANCE detection system Introduction: Accurate, cost-effective methods for testing anaplas- (Dako) and FISH with dual-color, break-apart probe (Abbott Mo- tic lymphoma kinase gene rearrangement (ALK) are needed to lecular) on formalin-fixed, paraffin-embedded tissue. select patients with non-small cell lung carcinoma for ALK-inhibitor Results: Cases were assessed as IHC score 0 (no staining; n  69), therapy. Fluorescent in situ hybridization (FISH) is used to detect 1 (faint cytoplasmic staining, n  21), 2 (moderate, smooth ALK, but it is expensive and not routinely available. We explored cytoplasmic staining; n  3), or 3 (intense, granular cytoplasmic the potential of an immunohistochemistry (IHC) scoring system as staining in 10% of tumor cells; n  8). All IHC 3 cases were an affordable, accessible http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Thoracic Oncology Wolters Kluwer Health

Correlation of IHC and FISH for ALK IHC Score Algorithm for FISH

Yang, Ping
Journal of Thoracic Oncology , Volume 6 (3) – Mar 1, 2011
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ISSN
1556-0864
DOI
10.1097/JTO.0b013e318209edb9
pmid
21278610
Publisher site
See Article on Publisher Site

Abstract

ORIGINAL ARTICLE Correlation of IHC and FISH for ALK Gene Rearrangement in Non-small Cell Lung Carcinoma IHC Score Algorithm for FISH Eunhee S. Yi, MD,* Jennifer M. Boland, MD,* Joseph J. Maleszewski, MD,* Anja C. Roden, MD,* Andre M. Oliveira, MD,* Marie-Christine Aubry, MD,* Michele R. Erickson-Johnson, MS,* Bolette L. Caron, BA,* Yan Li, MD,† Hui Tang, PhD,‡ Shawn Stoddard, RN,† Jason Wampfler, BS,‡ Kimary Kulig, PhD, MPH,§ and Ping Yang, MD, PhD† ALK1 monoclonal antibody with ADVANCE detection system Introduction: Accurate, cost-effective methods for testing anaplas- (Dako) and FISH with dual-color, break-apart probe (Abbott Mo- tic lymphoma kinase gene rearrangement (ALK) are needed to lecular) on formalin-fixed, paraffin-embedded tissue. select patients with non-small cell lung carcinoma for ALK-inhibitor Results: Cases were assessed as IHC score 0 (no staining; n  69), therapy. Fluorescent in situ hybridization (FISH) is used to detect 1 (faint cytoplasmic staining, n  21), 2 (moderate, smooth ALK, but it is expensive and not routinely available. We explored cytoplasmic staining; n  3), or 3 (intense, granular cytoplasmic the potential of an immunohistochemistry (IHC) scoring system as staining in 10% of tumor cells; n  8). All IHC 3 cases were an affordable, accessible

Journal

Journal of Thoracic Oncology – Wolters Kluwer Health

Published: Mar 1, 2011

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