Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Characterization and Applications of a Newly Developed Rabbit Monoclonal Antibody to Cytokeratin 7 (CK7) for Immunohistochemistry

Characterization and Applications of a Newly Developed Rabbit Monoclonal Antibody to Cytokeratin... Downloaded from http://journals.lww.com/appliedimmunohist by BhDMf5ePHKbH4TTImqenVA5KvPVPZ0P5BEgU+IUTEfzO/GUWifn2IfwcEVVH9SSn on 06/10/2020 TECHNICAL ARTICLE Characterization and Applications of a Newly Developed Rabbit Monoclonal Antibody to Cytokeratin 7 (CK7) for Immunohistochemistry Weimin Qi, MD, PhD, Joseph Chu, BS, Ding Zhou, BS, and David Tacha, PhD urothelium, but not in most colon cancers or stratified 4,5 Abstract: In this paper, we describe the development of a novel squamous epithelium. It is particularly useful in rabbit monoclonal cytokeratin 7 (CK7) antibody. CK7 is widely combination with other antibodies like TTF1, CK20, or used in immunohistochemical tests that are commonly per- CDX2 to distinguish cancer of unknown primary like formed in histopathology laboratories. Rabbits were immunized breast, lung, ovary, and urothelium from clone meta- 6–9 with a peptide corresponding to C-terminus of CK7. Recombi- stasis. CDX2 is preferred over CK20 due to the fact that nant monoclonal antibodies were developed using proprietary it is a highly sensitive and specific maker of adenocarci- technology. Antibodies were screened by enzyme-linked noma of intestinal origin and is rarely seen in tumor from immunosorbent assay against CK7 peptide, and the specificity other sites. Also, CDX2 is not expressed in mucinous of the antibody was verified by Western blotting on Hela cell bronchioloalveolar http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied Immunohistochemistry & Molecular Morphology Wolters Kluwer Health

Characterization and Applications of a Newly Developed Rabbit Monoclonal Antibody to Cytokeratin 7 (CK7) for Immunohistochemistry

Loading next page...
 
/lp/wolters-kluwer-health/characterization-and-applications-of-a-newly-developed-rabbit-18DRXKhIVV
Publisher
Wolters Kluwer Health
ISSN
1541-2016
DOI
10.1097/PAI.0b013e3181917af0
Publisher site
See Article on Publisher Site

Abstract

Downloaded from http://journals.lww.com/appliedimmunohist by BhDMf5ePHKbH4TTImqenVA5KvPVPZ0P5BEgU+IUTEfzO/GUWifn2IfwcEVVH9SSn on 06/10/2020 TECHNICAL ARTICLE Characterization and Applications of a Newly Developed Rabbit Monoclonal Antibody to Cytokeratin 7 (CK7) for Immunohistochemistry Weimin Qi, MD, PhD, Joseph Chu, BS, Ding Zhou, BS, and David Tacha, PhD urothelium, but not in most colon cancers or stratified 4,5 Abstract: In this paper, we describe the development of a novel squamous epithelium. It is particularly useful in rabbit monoclonal cytokeratin 7 (CK7) antibody. CK7 is widely combination with other antibodies like TTF1, CK20, or used in immunohistochemical tests that are commonly per- CDX2 to distinguish cancer of unknown primary like formed in histopathology laboratories. Rabbits were immunized breast, lung, ovary, and urothelium from clone meta- 6–9 with a peptide corresponding to C-terminus of CK7. Recombi- stasis. CDX2 is preferred over CK20 due to the fact that nant monoclonal antibodies were developed using proprietary it is a highly sensitive and specific maker of adenocarci- technology. Antibodies were screened by enzyme-linked noma of intestinal origin and is rarely seen in tumor from immunosorbent assay against CK7 peptide, and the specificity other sites. Also, CDX2 is not expressed in mucinous of the antibody was verified by Western blotting on Hela cell bronchioloalveolar

Journal

Applied Immunohistochemistry & Molecular MorphologyWolters Kluwer Health

Published: May 1, 2009

There are no references for this article.