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Downloaded from http://journals.lww.com/appliedimmunohist by BhDMf5ePHKbH4TTImqenVA5KvPVPZ0P5BEgU+IUTEfzO/GUWifn2IfwcEVVH9SSn on 06/10/2020 RESEARCH ARTICLE Application of “In Vivo Cryotechnique” to Morphological and Immunohistochemical Analyses of Living Mouse Subepicardial Microcirculation Under Various Pathological Conditions Liye Shi, MD, PhD,* Zilong Li, MD, PhD,w Xiaoyue Zhai, MD, PhD,z Bin Ning, BSc,z Bei Yang, PhD,z and Guoxian Qi, MD, PhD* Key Words: subepicardial microcirculation, erythrocyte mor- Abstract: “In vivo cryotechnique” (IVCT), which involves phology, microvasculature permeability, various pathological immediately cryofixing cells and tissues of living animals in conditions, in vivo cryotechnique situ, can display more native morphology in vivo and eliminate (Appl Immunohistochem Mol Morphol 2012;20:304–317) artificial changes in conventional preparations. However, the technical characteristics of IVCT are not known for the practical examination of subepicardial microcirculation of beating heart tissue. Histological sections of subepicardial area were prepared bnormal microcirculation is an important feature of using IVCT and conventional fixation methods: quick freezing, Amany cardiac diseases. Pathological studies of cardiac immersion fixation, or perfusion fixation followed by alcohol microcirculation have been typically carried out in paraffin dehydration, respectively from healthy mice. In addition, sections prepared by conventional fixation methods, changes of erythrocyte shape, T-tubule, and microvasculature including quick freezing (QF), immersion fixation, or per- in mouse heart from
Applied Immunohistochemistry & Molecular Morphology – Wolters Kluwer Health
Published: May 1, 2012
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