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TECHNICAL ARTICLE A Simple Method for Generating Multitissue Blocks Without Special Equipment Markku Miettinen, MD based on encasing tissue pieces by amniotic membrane to Abstract: The idea of multitumor block to expedite simultaneous create a sausage-like block. Battifora further developed analysis of multiple tissue specimens was pioneered by Battifora, the multitissue block to be geographically configured in a and several variations have been published since then. More “checker-board” manner, to include precise identification recently, microarray technology has been introduced to allow of individual pieces thus allowing efficient use in clinical placement of up to several hundreds specimens in 1 block using research of tumors and antibody development. Various manual or automated sampling devices. This paper reports a applications of the same theme have been subsequently 3,4 5 manual technique for preparation of a multitissue block. Gen- described. More recently, Kononen et al have devel- eration of such blocks requires no special equipment, and flex- oped a tissue array based on needle core sampling of ible block design is possible depending on nature of available donor blocks inserted in customized holes made in re- material and desired sample size. The first step is dissection of cipient blocks. This technique, either
Applied Immunohistochemistry & Molecular Morphology – Wolters Kluwer Health
Published: Jul 1, 2012
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