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A Microwave Technique for Double Indirect Immunostaining of Human Brain Tissue Cultures With Mouse Monoclonal Antibodies

A Microwave Technique for Double Indirect Immunostaining of Human Brain Tissue Cultures With... RESEARCH ARTICLE A Microwave Technique for Double Indirect Immunostaining of Human Brain Tissue Cultures With Mouse Monoclonal Antibodies Nicholas E. Jufas, BMedSc, Ben Roediger, BSc (Hons), and Patricia J. Armati, MSc, PhD it could not be recognized with conventional detection Abstract: The use of 2 monoclonal antibodies during double systems. They used the highly sensitive biotin-tyramide immunohistochemistry would enable the use of a greater variety amplification system to detect the first antigen. The of antibody combinations. Here, we demonstrate a simple, cost second antigen was then detected by conventional effective method of double indirect immunostaining of cultured immunostaining. However, the use of this technique cells using primary antibodies from the same species. This may be precluded by variables, such as only the biotin- method uses microwaving of cell samples immediately after the tyramide system being able to detect primary antibodies application of the first secondary antibody, and significantly expressed at low levels. reduces the level of nonspecific staining. This technique does not Recently, there has been increased interest in the elute the antibodies, nor raise the sample temperature above utilization of microwaves for immunohistochemistry. To 371C. obtain a reaction product, reagents must diffuse into the tissue or cell culture http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied Immunohistochemistry & Molecular Morphology Wolters Kluwer Health

A Microwave Technique for Double Indirect Immunostaining of Human Brain Tissue Cultures With Mouse Monoclonal Antibodies

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ISSN
1541-2016
DOI
10.1097/PAI.0b013e31802eeb23
pmid
18091314
Publisher site
See Article on Publisher Site

Abstract

RESEARCH ARTICLE A Microwave Technique for Double Indirect Immunostaining of Human Brain Tissue Cultures With Mouse Monoclonal Antibodies Nicholas E. Jufas, BMedSc, Ben Roediger, BSc (Hons), and Patricia J. Armati, MSc, PhD it could not be recognized with conventional detection Abstract: The use of 2 monoclonal antibodies during double systems. They used the highly sensitive biotin-tyramide immunohistochemistry would enable the use of a greater variety amplification system to detect the first antigen. The of antibody combinations. Here, we demonstrate a simple, cost second antigen was then detected by conventional effective method of double indirect immunostaining of cultured immunostaining. However, the use of this technique cells using primary antibodies from the same species. This may be precluded by variables, such as only the biotin- method uses microwaving of cell samples immediately after the tyramide system being able to detect primary antibodies application of the first secondary antibody, and significantly expressed at low levels. reduces the level of nonspecific staining. This technique does not Recently, there has been increased interest in the elute the antibodies, nor raise the sample temperature above utilization of microwaves for immunohistochemistry. To 371C. obtain a reaction product, reagents must diffuse into the tissue or cell culture

Journal

Applied Immunohistochemistry & Molecular Morphology – Wolters Kluwer Health

Published: Jan 1, 2008

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