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A Home-brew Real-time PCR Assay for Reliable Detection and Quantification of Mature miR-122

A Home-brew Real-time PCR Assay for Reliable Detection and Quantification of Mature miR-122 RESEARCH ARTICLE A Home-brew Real-time PCR Assay for Reliable Detection and Quantification of Mature miR-122 Mahmood Naderi,* Hossein Abdul Tehrani, PhD,* Masoud Soleimani, PhD,w Iman Shabani, PhD,z and Seyed Mahmoud Hashemi, PhDy iRNAs are small noncoding RNAs of 22 nucleo- Abstract: miR-122 is a liver-specific miRNA that has significant Mtides in length that bind to their target sites on the 3 gene expression alterations in response to specific pathophysio- UTR region of mRNAs and subsequently repress gene logical circumstances of liver such as drug-induced liver injury, expression. Among miRNAs, miR-122 is one of the tis- hepatocellular carcinoma, and hepatitis B and C virus infections. sue-specific miRNAs, accounting for 70% of the miRNA Therefore, accurate and precise quantification of miR-122 is very pool in the liver. This miRNA is transcribed from a important for clinical diagnostics. However, because of the lack single locus on chromosome 18 in humans. Interestingly, of in vitro diagnostics assays for miR-122 detection and quan- the sequence of mature miR-122 is completely preserved tification of the existence of an open-source assay could in- among all the species in which it has been detected, in- evitably provide external evaluation by other researchers and the dicating that the http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied Immunohistochemistry & Molecular Morphology Wolters Kluwer Health

A Home-brew Real-time PCR Assay for Reliable Detection and Quantification of Mature miR-122

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Copyright
Copyright © 2014 Wolters Kluwer Health, Inc. All rights reserved.
ISSN
1541-2016
DOI
10.1097/PAI.0000000000000125
pmid
25075470
Publisher site
See Article on Publisher Site

Abstract

RESEARCH ARTICLE A Home-brew Real-time PCR Assay for Reliable Detection and Quantification of Mature miR-122 Mahmood Naderi,* Hossein Abdul Tehrani, PhD,* Masoud Soleimani, PhD,w Iman Shabani, PhD,z and Seyed Mahmoud Hashemi, PhDy iRNAs are small noncoding RNAs of 22 nucleo- Abstract: miR-122 is a liver-specific miRNA that has significant Mtides in length that bind to their target sites on the 3 gene expression alterations in response to specific pathophysio- UTR region of mRNAs and subsequently repress gene logical circumstances of liver such as drug-induced liver injury, expression. Among miRNAs, miR-122 is one of the tis- hepatocellular carcinoma, and hepatitis B and C virus infections. sue-specific miRNAs, accounting for 70% of the miRNA Therefore, accurate and precise quantification of miR-122 is very pool in the liver. This miRNA is transcribed from a important for clinical diagnostics. However, because of the lack single locus on chromosome 18 in humans. Interestingly, of in vitro diagnostics assays for miR-122 detection and quan- the sequence of mature miR-122 is completely preserved tification of the existence of an open-source assay could in- among all the species in which it has been detected, in- evitably provide external evaluation by other researchers and the dicating that the

Journal

Applied Immunohistochemistry & Molecular Morphology – Wolters Kluwer Health

Published: Sep 1, 2015

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