Access the full text.
Sign up today, get DeepDyve free for 14 days.
Loading next page...
/lp/wiley/xenotransplantation-literature-update-september-october-2016-BEG6IYWDnJ
References (8)
-
Yi Li, Yujia Wang, Qiong Wu, Li Li, Yujun Shi, H. Bu, J. Bao (2016)
Comparison of methods for isolating primary hepatocytes from mini pigsXenotransplantation, 23
-
Anna Buermann, D. Römermann, W. Baars, J. Hundrieser, J. Klempnauer, R. Schwinzer (2016)
Inhibition of B‐cell activation and antibody production by triggering inhibitory signals via the PD‐1/PD‐ligand pathwayXenotransplantation, 23
-
E. Reuven, Shani Ben-Arye, Tal Marshanski, M. Breimer, Hai Yu, Imen Fellah-Hebia, J. Roussel, C. Costa, M. Galiñanes, R. Mañez, T. Tourneau, J. Soulillou, E. Cozzi, Xi Chen, Vered Padler-Karavani (2016)
Characterization of immunogenic Neu5Gc in bioprosthetic heart valvesXenotransplantation, 23
-
Whayoung Lee, Cassandra Long, J. Ramsoondar, D. Ayares, D. Cooper, R. Manji, H. Hara (2016)
Human antibody recognition of xenogeneic antigens (NeuGc and Gal) on porcine heart valves: could genetically modified pig heart valves reduce structural valve deterioration?Xenotransplantation, 23
-
T. Itoh, Y. Hata, Hitomi Nishinakamura, K. Kumano, Hiroyuki Takahashi, S. Kodama (2016)
Islet‐derived damage‐associated molecular pattern molecule contributes to immune responses following microencapsulated neonatal porcine islet xenotransplantation in miceXenotransplantation, 23
-
S. Ock, Jungkyu Lee, K. Oh, Seongsoo Hwang, I. Yun, C. Ahn, H. Chee, Hwajung Kim, J. Park, Sung Kim, Youngim Kim, G. Im, E. Park (2016)
Molecular immunology profiles of monkeys following xenografting with the islets and heart of α‐1,3‐galactosyltransferase knockout pigsXenotransplantation, 23
-
B. Petersen, A. Frenzel, A. Lucas-Hahn, D. Herrmann, P. Hassel, S. Klein, Maren Ziegler, K. Hadeler, H. Niemann (2016)
Efficient production of biallelic GGTA1 knockout pigs by cytoplasmic microinjection of CRISPR/Cas9 into zygotesXenotransplantation, 23
-
S. Heinke, B. Ludwig, Undine Schubert, Janine Schmid, T. Kiss, A. Steffen, S. Bornstein, S. Ludwig (2016)
Diabetes induction by total pancreatectomy in minipigs with simultaneous splenectomy: a feasible approach for advanced diabetes researchXenotransplantation, 23
- Publisher
- Wiley
- Copyright
- © 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
- ISSN
- 0908-665X
- eISSN
- 1399-3089
- DOI
- 10.1111/xen.12281
- pmid
- 27897334
- Publisher site
- See Article on Publisher Site
Abstract
Received:Nov42016 Accepted:Nov72016 DOI: 10.1111/xen.12281 LITERA TURE UPD A TE Xenotransplantation literature update, September–October AnAn Hua | Magie Steinhoff | Christopher Burlak SchultzDiabetesInstitute,Departmentof Surgery,UniversityofMinnesotaSchoolofMedicine,Minneapolis,Minnesota CorrespondenceChristopherBurlak,SchultzDiabetesInstitute,Departmentof Surgery,UniversityofMinnesotaSchoolofMedicine,Minneapolis,MN,USA. Email:christopherburlak@gmail.com KEYWORDS:CRISPR,diabetes,heart,Neu5Gc Theintroduction ofCRISPR/Cas9inxenotransplantation hasgreatly wild-type(WT)pigsforheartvalvereplacement.Leeetal. demon- enhancedtherateandefficiency intheproduction ofKOporcinecells. stratedtheirprogressintheproduction of1,3-galactosyltransferase Peterson etal. have demonstrated the effectiveness of CRISPR/ geneknockout(GTKO)andGTKO/N-glycolylneuraminicacidgene Cas9throughthegeneration ofsixhealthyKOpiglets;fourofthesix knockout (GTKO/NeuGcKO) pigs through phenotypic screening. live-bornpigletspresentednegative for1,3-galactosyltransferaseby Reducedantibody bindingtoGTKOandGTKO/NeuGcKOGBHVs flow cytometryanalysis.Theseresultscommunicatethecapacityof comparedtoWTGBHVssubstantiates thepreferenceforKOporcine CRISPR/Cas9technologytobeusedforintracytoplasmicmicroinjec- heartvalves. tion ofporcinezygotesasasubstitute forsomatic cellnucleartransfer Successinxenografts iscontributedtoadvancesinthecharac- (SCNT)andmRNAinjections. terization oftheimmunogenicsialicacid:N-glycolylneuraminicacid Presenceofallo-andxenografts provokeB-cellactivation and (Neu5Gc). Reuven etal. show and quantify Neu5Gc expression proliferation ofdonor-reactive antibodies thatgreatlyreduceitslong- inporcineandbovinecardiactissues andcommercialBHVusedin termefficacy. Buermannetal. investigated theresponseofB-cell clinics.Identification andcharacterization ofxeno-antigens aidinthe activity throughincreasedstimulation oftheco-inhibitoryreceptor selectionandmodificationof KOorgans. programmedcelldeath-1(PD-1;CD279),byrecombinantagonistic Microencapsulatedneonatalporcineislets(NPI)couldprovidean solubleligandsorL23transfectantsoverexpressingthemembrane- alternative toallotransplantation andalsohelptoshieldthetrans- boundhumanPD-L1.Resultsdemonstrateddownregulation ofanti- plantedcellsfromthehumanimmunesystem;previousclinicaltrials bodyproduction whenexposedtoselectamountsofligandsbinding haveshownNPItoeectiv ff ely lowerA1clevelsanddecreaseoccur- tothePD-1receptor.Identification ofinhibitorysignalsisneces- rencesofhypoglycemia.However,thereisasignificant lossinefficacy saryfortheselection ofimmunosuppressivedrugsinthesuccessof whentheNPIaretransplantedintotheperitonealcavity,whichisdue transplantation. tolocalizedinflammation. Itohetal. demonstratedthattheNPIcould Developments of non-in vasive and time- efficient methods are regaintheirefficacy throughtargettreatmentoftheinflammation critical indetecting earlysignsofxenograft failure.Ocketal. have usinganti- TNF-alphainthemousemodel. recently developed a method in real time for evaluating immune Akeyfactorinanypreclinicaldiabetestreatmentstudyisananimal system-relatedgenesinbloodofrecipientcynomologousmonkeysbe- model.Itisimportanttoinducediabetesintheanimalmodelinaway foreandaerft transplantation. Recipientsweretransplantedwithheart thatissafe,reproducible,andreliable.ThatiswhyHeinkeetal.have orpancreatic isletsfromsingleanddouble1,3-galactosyltransferase induceddiabetesmellitusinminipigsthroughpancreatectomyand (GalT)knockout(KO)pigs.Throughphenotypicanalysisofalpha-Gal splenectomy.Thismethodleavesthepigscompletelyinsulindeficient epitopebyflow cytometryandRT PCRarrays,Ocketal.wereableto withnoclinicalsymptomsduetothelossofexocrinefunction. Heinke detectearlysignsofgraft failure.Thisnovelmethodcanhelpprolong etal. demonstratedthatthismethodcouldreplaceothersduetothe thesuccessofxenografts andaidintheselection ofimmunosuppres- factthatitwouldnotimpactotherorgansystems,suchaschemical sivedrugs. induction,anditiseectiv ff eandreliable. Shortage of human hearts has necessitated our dependence The need for xenotransplantation of hepatocytes
Journal
Xenotransplantation – Wiley
Published: Nov 1, 2016
Keywords: ; ; ;