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The influence of intravenous hydrocortisone on cytokine levels in children with asthma

The influence of intravenous hydrocortisone on cytokine levels in children with asthma Nocturnal airway obstruction occurs frequently in childhood asthma and results from increased airway inflammation. Lymphocytes are believed to be key effector cells of airway wall inflammation, releasing pro‐inflammatory mediators and cytokines. A previous study showed that hydrocortisone infusion, an effective anti‐inflammatory treatment, improves nocturnal and daytime FEV1 values. This study in 16 children with moderate asthma was designed to assess whether there exists day and night differences in IL‐4, IL‐5, IL‐8, and IFN‐γ production of concanavaline A stimulated peripheral blood mononuclear cells. Furthermore, we investigated whether substitution of low serum cortisol levels with intravenous hydrocortisone would affect those parameters. Saline (as a placebo) or hydrocortisone (30 μg/m2 body surface area/24 h) was intravenously administered in a randomized, double blind, cross‐over design. Measurements under saline or hydrocortisone infusions were separated by 1 wk. At 04:00 and 16:00 hours 10 ml blood was taken for determination of peripheral blood mononuclear cell isolation and stimulation, and an eosinophil count. Hydrocortisone infusion significantly reduced the nocturnal fall in FEV1. Median values of IFNγ, IL‐4, IL‐5, and IL‐8 produced by peripheral blood mononuclear cells did not significantly differ at 04:00 and 16:00 hours, both with saline and hydrocortisone infusion. Our results suggest that FEV1 improvement is not due to suppression of circulating peripheral blood mononuclear cell activation. We hypothesize that it is rather due to its effect on local lung tissue epithelial and/or fibroblasts thereby reducing airway inflammation and vascular leakage. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Pediatric Allergy and Immunology Wiley

The influence of intravenous hydrocortisone on cytokine levels in children with asthma

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References (36)

Publisher
Wiley
Copyright
Copyright © 2005 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0905-6157
eISSN
1399-3038
DOI
10.1111/j.1399-3038.2005.00282.x
pmid
15943592
Publisher site
See Article on Publisher Site

Abstract

Nocturnal airway obstruction occurs frequently in childhood asthma and results from increased airway inflammation. Lymphocytes are believed to be key effector cells of airway wall inflammation, releasing pro‐inflammatory mediators and cytokines. A previous study showed that hydrocortisone infusion, an effective anti‐inflammatory treatment, improves nocturnal and daytime FEV1 values. This study in 16 children with moderate asthma was designed to assess whether there exists day and night differences in IL‐4, IL‐5, IL‐8, and IFN‐γ production of concanavaline A stimulated peripheral blood mononuclear cells. Furthermore, we investigated whether substitution of low serum cortisol levels with intravenous hydrocortisone would affect those parameters. Saline (as a placebo) or hydrocortisone (30 μg/m2 body surface area/24 h) was intravenously administered in a randomized, double blind, cross‐over design. Measurements under saline or hydrocortisone infusions were separated by 1 wk. At 04:00 and 16:00 hours 10 ml blood was taken for determination of peripheral blood mononuclear cell isolation and stimulation, and an eosinophil count. Hydrocortisone infusion significantly reduced the nocturnal fall in FEV1. Median values of IFNγ, IL‐4, IL‐5, and IL‐8 produced by peripheral blood mononuclear cells did not significantly differ at 04:00 and 16:00 hours, both with saline and hydrocortisone infusion. Our results suggest that FEV1 improvement is not due to suppression of circulating peripheral blood mononuclear cell activation. We hypothesize that it is rather due to its effect on local lung tissue epithelial and/or fibroblasts thereby reducing airway inflammation and vascular leakage.

Journal

Pediatric Allergy and Immunology – Wiley

Published: Jun 1, 2005

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