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Reactivity of Human Natural Antibodies to Endothelial Cells From Gal&agr;(1,3)Gal-Deficient PigsTransplantation, 83
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Erythrocytes from GGTA1/CMAH knockout pigs: implications for xenotransfusion and testing in non‐human primatesXenotransplantation, 21
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Genetically engineered pig red blood cells for clinical transfusion: initial in vitro studiesTransfusion, 49
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Silencing the porcine iGb3s gene does not affect Galα3Gal levels or measures of anticipated pig‐to‐human and pig‐to‐primate acute rejectionXenotransplantation, 23
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Double knockout pigs deficient in N‐glycolylneuraminic acid and Galactose α‐1,3‐Galactose reduce the humoral barrier to xenotransplantationXenotransplantation, 20
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Thyroid hormone: relevance to xenotransplantationXenotransplantation, 23
Whayoung Lee, H. Hara, M. Ezzelarab, H. Iwase, R. Bottino, Cassandra Long, J. Ramsoondar, D. Ayares, D. Cooper (2016)
Initial in vitro studies on tissues and cells from GTKO/CD46/NeuGcKO pigsXenotransplantation, 23
PhD Verginella, PhD Vodopivec, PhD Wakounig, Javier Domingo, Luigi Finocchietti, Vesna ć, Le e, la, Gary Cohen (1917)
Historical ReviewActa Radiologica, 3
(2017)
The impact of serum incubation time on IgM/IgG binding to porcine aortic endothelial cells
B. Wong, Kazuhiko Yamada, M. Okumi, J. Weiner, P. O'Malley, Y. Tseng, F. Dor, D. Cooper, S. Saidman, A. Griesemer, D. Sachs (2006)
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A. Azimzadeh, G. Byrne, M. Ezzelarab, E. Welty, G. Braileanu, Xiangfei Cheng, S. Robson, C. McGregor, D. Cooper, R. Pierson (2014)
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Fan Liang, I. Wamala, J. Scalea, A. Tena, T. Cormack, S. Pratts, R. Duran‐Struuck, N. Elias, M. Hertl, Christene Huang, D. Sachs (2013)
Increased levels of anti‐non‐Gal IgG following pig‐to‐baboon bone marrow transplantation correlate with failure of engraftmentXenotransplantation, 20
Min Zhu, Wei Zhang, Fang Liu, Lu Wang, B. Liu, Dong-Wen Chen, Xuejun Zhu, Weijie Zhang, T. Ichim, Zhishui Chen, P. Zhou, Shi Chen, Gang Chen (2008)
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M. Ezzelarab, H. Hara, J. Busch, P. Rood, Xiaocheng Zhu, Z. Ibrahim, S. Ball, D. Ayares, M. Awwad, D. Cooper (2006)
Antibodies directed to pig non‐Gal antigens in naïve and sensitized baboonsXenotransplantation, 13
The results of the assay for measuring anti‐non‐Gal antibodies (which affect pig xenograft survival) in recipients are important. Serum incubation time and concentration may be important factors in the extent of antibody binding to the graft. The aim of this in vitro study was to determine the optimal incubation time and serum concentration for measuring anti‐non‐Gal antibody binding to porcine aortic endothelial cells (pAECs). Pooled human, naive, and sensitized baboon sera were incubated with wild‐type, α1,3‐galactosyltransferase gene‐knockout (GTKO), and GTKO/human CD55 pAECs. IgM/IgG binding to pAECs after varying serum incubation times (0.5, 1, 2, and 3 hour) and concentrations (5, 10, 20, and 40 μL) was determined by flow cytometry. An increase in incubation time from 30 minutes to 2 hour was associated with increases in anti‐non‐Gal IgM/IgG binding to GTKO and GTKO/hCD55 pAECs of pooled human, naive and sensitized baboon sera (P<.05). Pooled human serum showed a significant increase in anti‐non‐Gal IgM (1.5 times) and a minimal increase in anti‐non‐Gal IgG antibody binding. IgM/IgG binding of sensitized baboon serum to GTKO pAECs after 2‐hour incubation was 1.5 times and 2 times greater than after 30‐minutes incubation, respectively, whereas naïve baboon sera showed minimal (non‐significant) increase in anti‐non‐Gal IgM/IgG antibody binding. With 2‐hour incubation, increasing the serum concentration from 5 μL to 20 μL significantly increased antibody binding to non‐Gal antigens in pooled human and sensitized baboon serum. With naïve baboon serum, only IgG was significantly increased. Increasing the serum incubation time contributed to improve the sensitivity of detecting anti‐non‐Gal antibodies, without affecting cell viability in vitro.
Xenotransplantation – Wiley
Published: Jul 1, 2017
Keywords: ; ; ; ; ; ;
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