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THE GENETICAL SOCIETY

THE GENETICAL SOCIETY STUDIES ON HETEROGENEOUS CLONES (HETEROCLONES) IN STREPTOM YCES COELICOLOR G. SERMONTI, Istituto Superiore di SanitB, Rome, and D. A. HOPWOOD, Botany School, University of Cambridge Some of the recombinant colonies which develop on selective media after plating spores from a mixed culture of two complementary auxotrophic strains of Streptomyces coelicolor turn out to contain a mixture of parental and recombinant phenotypes. The hypothesie is put forward that these heterogenous colonies (heteroclones) develop from plating-units containing a highly unstable heterozygous nucleus which undergoes segregation during the development of the colony. Some of the parental markers appear amongst the segregants with reduced frequencies, or are even lacking altogether. With this limitation, the genotypes and frequencies of the segregants are predictable on the basis of the linkage relations of the markers deduced from standard selective analysis. The observed deficiency of certain markers is interpreted as being due to hemizygosity of some loci in the heterozygous plating-units. Pre-zygotic elimination has not been critically shown; it is evident, however, that extensive post-zygotic elimination occurs. Genetic analysis of the heteroclones has confirmed the location of several markers in two linkage groups, and permitted the mapping of new mutations. The heteroclones provide a convenient complementation http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Annals of Human Genetics Wiley

THE GENETICAL SOCIETY

Annals of Human Genetics , Volume 25 (4) – May 1, 1962
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Publisher
Wiley
Copyright
Copyright © 1962 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0003-4800
eISSN
1469-1809
DOI
10.1111/j.1469-1809.1962.tb01763.x
Publisher site
See Article on Publisher Site

Abstract

STUDIES ON HETEROGENEOUS CLONES (HETEROCLONES) IN STREPTOM YCES COELICOLOR G. SERMONTI, Istituto Superiore di SanitB, Rome, and D. A. HOPWOOD, Botany School, University of Cambridge Some of the recombinant colonies which develop on selective media after plating spores from a mixed culture of two complementary auxotrophic strains of Streptomyces coelicolor turn out to contain a mixture of parental and recombinant phenotypes. The hypothesie is put forward that these heterogenous colonies (heteroclones) develop from plating-units containing a highly unstable heterozygous nucleus which undergoes segregation during the development of the colony. Some of the parental markers appear amongst the segregants with reduced frequencies, or are even lacking altogether. With this limitation, the genotypes and frequencies of the segregants are predictable on the basis of the linkage relations of the markers deduced from standard selective analysis. The observed deficiency of certain markers is interpreted as being due to hemizygosity of some loci in the heterozygous plating-units. Pre-zygotic elimination has not been critically shown; it is evident, however, that extensive post-zygotic elimination occurs. Genetic analysis of the heteroclones has confirmed the location of several markers in two linkage groups, and permitted the mapping of new mutations. The heteroclones provide a convenient complementation

Journal

Annals of Human Genetics – Wiley

Published: May 1, 1962

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