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Iris Meisen, J. Peter-Katalinic, J. Müthing (2003)
Discrimination of neolacto-series gangliosides with alpha2-3- and alpha2-6-linked N-acetylneuraminic acid by nanoelectrospray ionization low-energy collision-induced dissociation tandem quadrupole TOF MS.Analytical chemistry, 75 21
J. Baenziger, D. Fiete (1979)
Structure of the complex oligosaccharides of fetuin.The Journal of biological chemistry, 254 3
Yawei Xu, Zhangxiong Wu, Lijuan Zhang, Haojie Lu, Pengyuan Yang, P. Webley, Dongyuan Zhao (2009)
Highly specific enrichment of glycopeptides using boronic acid-functionalized mesoporous silica.Analytical chemistry, 81 1
H. Geyer, R. Geyer (2006)
Strategies for analysis of glycoprotein glycosylation.Biochimica et biophysica acta, 1764 12
Jia Zhao, D. Simeone, D. Heidt, Michelle Anderson, D. Lubman (2006)
Comparative serum glycoproteomics using lectin selected sialic acid glycoproteins with mass spectrometric analysis: application to pancreatic cancer serum.Journal of proteome research, 5 7
Xin Liu, David McNally, H. Nothaft, C. Szymanski, J. Brisson, Jianjun Li (2006)
Mass spectrometry-based glycomics strategy for exploring N-linked glycosylation in eukaryotes and bacteria.Analytical chemistry, 78 17
J. Wohlgemuth, M. Karas, T. Eichhorn, R. Hendriks, S. Andrecht (2009)
Quantitative site-specific analysis of protein glycosylation by LC-MS using different glycopeptide-enrichment strategies.Analytical biochemistry, 395 2
M. Larsen, S. Jensen, L. Jakobsen, N. Heegaard (2007)
Exploring the Sialiome Using Titanium Dioxide Chromatography and Mass Spectrometry *SMolecular & Cellular Proteomics, 6
J. Irungu, Dilusha Dalpathado, Eden Go, Hui Jiang, H. Ha, G. Bousfield, H. Desaire (2006)
Method for characterizing sulfated glycoproteins in a glycosylation site-specific fashion, using ion pairing and tandem mass spectrometry.Analytical chemistry, 78 4
Jing Cao, Chengpin Shen, Hong Wang, Huali Shen, Yaohan Chen, Aiying Nie, Guo-quan Yan, Haojie Lu, Yin‐kun Liu, Pengyuan Yang (2009)
Identification of N-glycosylation sites on secreted proteins of human hepatocellular carcinoma cells with a complementary proteomics approach.Journal of proteome research, 8 2
M. Başoğlu, M. Yıldırgan, S. Taysı, I. Yilmaz, A. Kızıltunç, A. Balik, F. Celebi, S. Atamanalp (2003)
Levels of soluble intercellular adhesion molecule‐1 and total sialic acid in serum of patients with colorectal cancerJournal of Surgical Oncology, 83
R. Dwek (1996)
Glycobiology: Toward Understanding the Function of Sugars.Chemical reviews, 96 2
C. Temporini, E. Calleri, G. Massolini, G. Caccialanza (2008)
Integrated analytical strategies for the study of phosphorylation and glycosylation in proteins.Mass spectrometry reviews, 27 3
K. Kubota, Yuji Sato, Yusuke Suzuki, Naoko Goto-Inoue, T. Toda, Minoru Suzuki, S. Hisanaga, A. Suzuki, T. Endo (2008)
Analysis of glycopeptides using lectin affinity chromatography with MALDI-TOF mass spectrometry.Analytical chemistry, 80 10
A. Helenius, M. Aebi (2004)
Roles of N-linked glycans in the endoplasmic reticulum.Annual review of biochemistry, 73
T. Blake, Tracie Williams, J. Pirkle, J. Barr (2009)
Targeted N-linked glycosylation analysis of H5N1 influenza hemagglutinin by selective sample preparation and liquid chromatography/tandem mass spectrometry.Analytical chemistry, 81 8
C. Feijoo-Carnero, F. Rodríguez-Berrocal, M. Cadena, D. Ayude, A. Carlos, V. Martínez-Zorzano (2004)
Clinical Significance of Preoperative Serum Sialic Acid Levels in Colorectal Cancer: Utility in the Detection of Patients at High Risk of Tumor RecurrenceThe International Journal of Biological Markers, 19
P. Rudd, T. Elliott, P. Cresswell, I. Wilson, R. Dwek (2001)
Glycosylation and the immune system.Science, 291 5512
S. Gökmen, C. Kazezoğlu, B. Sunar, F. Özçelik, Özgül Güngör, F. Yorulmaz, Ş. Gülen (2006)
Relationship between serum sialic acids, sialic acid-rich inflammation-sensitive proteins and cell damage in patients with acute myocardial infarctionClinical Chemistry and Laboratory Medicine (CCLM), 44
G. Palmisano, S. Lendal, Kasper Engholm-Keller, R. Leth-Larsen, B. Parker, M. Larsen (2010)
Selective enrichment of sialic acid–containing glycopeptides using titanium dioxide chromatography with analysis by HILIC and mass spectrometryNature Protocols, 5
M. Larsen, T. Thingholm, O. Jensen, P. Roepstorff, T. Jørgensen (2005)
Highly Selective Enrichment of Phosphorylated Peptides from Peptide Mixtures Using Titanium Dioxide Microcolumns*Molecular & Cellular Proteomics, 4
M. Thaysen‐Andersen, Simon Mysling, P. Højrup (2009)
Site-specific glycoprofiling of N-linked glycopeptides using MALDI-TOF MS: strong correlation between signal strength and glycoform quantities.Analytical chemistry, 81 10
E. Green, G. Adelt, J. Baenziger, S. Wilson, H. Halbeek (1988)
The asparagine-linked oligosaccharides on bovine fetuin. Structural analysis of N-glycanase-released oligosaccharides by 500-megahertz 1H NMR spectroscopy.The Journal of biological chemistry, 263 34
Sialylation of glycoproteins is vital for the function or physicochemical properties of a protein. It becomes more and more important to develop approaches that can be used to efficiently isolate and identify sialylated glycopeptides or glycoproteins for monitoring changes in glycoproteome. In the present study, we analyze intact structures of the enriched sialylated glycopeptides of bovine fetuin by matrix‐assisted laser desorption/ionization‐tandem mass spectrometry (MALDI‐MS/MS), without any chemical derivation. The experimental data show that the optimal loading buffer for TiO2 as matrix is 80% acetonitrile/2% TFA (trifluoroacetic acid)/100 mg/mL DHB (2,5‐dihydroxybenzoic acid) which is also compatible with MALDI‐mass spectrometric analysis. This study indicates that the improved enrichment approach combined with MALDI‐MS/MS may be a powerful tool to analyze intact structures and components of the sialylated glycopeptides from complex peptide mixture.
Chinese Journal of Chemistry – Wiley
Published: Nov 1, 2011
Keywords: ; ; ;
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