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The crystal structure of the A4 isoform of photosynthetic glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH) from Arabidopsis thaliana, expressed in recombinant form and complexed with NAD, is reported. The crystals, which were grown in 2.4 M ammonium sulfate and 0.1 M sodium citrate, belonged to space group I222. The asymmetric unit includes ten subunits, i.e. two independent tetramers plus a dimer that generates a third tetramer by a crystallographic symmetry operation. The crystal structure was solved by molecular replacement and refined to an R factor of 23.7% and an Rfree factor of 28.9% at 2.6 Å resolution. In the final model, each subunit binds one NAD+ molecule and two sulfates, which occupy the Ps and the Pi anion‐binding sites. Detailed knowledge of this structure is instrumental for structural investigation of supramolecular complexes of A4‐GAPDH, phosphoribulokinase and CP12, which are involved in the regulation of photosynthesis in the model plant A. thaliana.
Acta Crystallographica Section F – Wiley
Published: Jun 1, 2010
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