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Philip Martin, C. Purcarea, Pengfei Zhang, Asmita Vaishnav, Sharon Sadecki, Hedeel Guy-Evans, David Evans, B. Edwards (2005)
The crystal structure of a novel, latent dihydroorotase from Aquifex aeolicus at 1.7A resolution.Journal of molecular biology, 348 3
Mihwa Lee, Camilla Chan, Stephen Graham, Richard Christopherson, J. Guss, M. Maher (2007)
Structures of ligand-free and inhibitor complexes of dihydroorotase from Escherichia coli: implications for loop movement in inhibitor design.Journal of molecular biology, 370 5
Mark Larkin, G. Blackshields, N. Brown, R. Chenna, P. McGettigan, Hamish McWilliam, F. Valentin, Iain Wallace, A. Wilm, R. Lopez, J. Thompson, T. Gibson, D. Higgins (2007)
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Dihydroorotase from the hyperthermophile Aquifex aeolicus is activated by stoichiometric association with aspartate transcarbamoylase and forms a one-pot reactor for pyrimidine biosynthesis.Biochemistry, 48 4
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Structural and functional analysis of two glutamate racemase isozymes from Bacillus anthracis and implications for inhibitor design.Journal of molecular biology, 371 5
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Mihwa Lee, Camilla Chan, J. Guss, R. Christopherson, M. Maher (2005)
Dihydroorotase from Escherichia coli: loop movement and cooperativity between subunits.Journal of molecular biology, 348 3
Mihwa Lee, M. Maher, R. Christopherson, J. Guss (2007)
Kinetic and structural analysis of mutant Escherichia coli dihydroorotases: a flexible loop stabilizes the transition state.Biochemistry, 46 37
Dihydroorotase (EC 3.5.2.3) catalyzes the reversible cyclization of N‐carbamoyl‐l‐aspartate to l‐dihydroorotate in the third step of the pyrimidine‐biosynthesis pathway in Bacillus anthracis. A comparison is made between the structures of dihydroorotase from four different organisms, including B. anthracis dihydroorotase, and reveals substantial variations in the active site, dimer interface and overall tertiary structure. These differences demonstrate the utility of exploring multiple structures of a molecular target as expressed from different organisms and how these differences can be exploited for structure‐based drug discovery.
Acta Crystallographica Section F – Wiley
Published: Nov 1, 2010
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