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Spermiation and sperm quality of European catfish ( Silurus glanis L.) after implantation of GnRH analogues and injection of carp pituitary extract

Spermiation and sperm quality of European catfish ( Silurus glanis L.) after implantation of GnRH... Summary Spermiation was stimulated in the European catfish Silurus glanis L. by injection of carp pituitary preparation (5 mg kg‐1 of body weight) and mammalian GnRH ((D‐Ala6) en‐RH ProNHEt) implanted at a dose of 75 μg and 150 μg per male, respectively. The total number of spermatozoa collected by daily sampling during 7 days post‐stimulation and expressed in millions per kg of body weight was 3.29 with carp pituitaries, 0.872 and 0.780 with 75 μg and 150 μg GnRH anjoue implant, respectively, and 0.387 in the control group treated with Ringer solution. In all groups, after sperm stripping there was a spontaneous activation by urine of the sermatozoa motility which was revented by collection of the sperm in an immobilizing solution (NaCl 200 mM, Tris 30 mM, pH 7). The percentage of motile spermatozoa ranged from 80 to 65% at days 1 to 5, and less than 50% at day 6 and 7. A similar decline of quality was observed with time for mass progressive movement (55 to 65 s on day 1, and 28 to 35 s on day 7) and total duration of displacement (66 to 77 s on day 1, and 45 to 53 on day 7). http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Applied Ichthyology Wiley

Spermiation and sperm quality of European catfish ( Silurus glanis L.) after implantation of GnRH analogues and injection of carp pituitary extract

Linhart, O.; Billard, R.
Journal of Applied Ichthyology , Volume 10 (2‐3) – Oct 1, 1994
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References (4)

Publisher
Wiley
Copyright
Copyright © 1994 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0175-8659
eISSN
1439-0426
DOI
10.1111/j.1439-0426.1994.tb00157.x
Publisher site
See Article on Publisher Site

Abstract

Summary Spermiation was stimulated in the European catfish Silurus glanis L. by injection of carp pituitary preparation (5 mg kg‐1 of body weight) and mammalian GnRH ((D‐Ala6) en‐RH ProNHEt) implanted at a dose of 75 μg and 150 μg per male, respectively. The total number of spermatozoa collected by daily sampling during 7 days post‐stimulation and expressed in millions per kg of body weight was 3.29 with carp pituitaries, 0.872 and 0.780 with 75 μg and 150 μg GnRH anjoue implant, respectively, and 0.387 in the control group treated with Ringer solution. In all groups, after sperm stripping there was a spontaneous activation by urine of the sermatozoa motility which was revented by collection of the sperm in an immobilizing solution (NaCl 200 mM, Tris 30 mM, pH 7). The percentage of motile spermatozoa ranged from 80 to 65% at days 1 to 5, and less than 50% at day 6 and 7. A similar decline of quality was observed with time for mass progressive movement (55 to 65 s on day 1, and 28 to 35 s on day 7) and total duration of displacement (66 to 77 s on day 1, and 45 to 53 on day 7).

Journal

Journal of Applied Ichthyology – Wiley

Published: Oct 1, 1994

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