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Significance of an In Vivo Genotoxicity Test for Surface Waters Is Increased when the Physiological Characteristics of the Test Organism (Bivalvia: Mollusca) Are Taken into Account?

Significance of an In Vivo Genotoxicity Test for Surface Waters Is Increased when the... The alkaline filter elution assay using the gills of the freshwater clam species Corbicula fluminea detects breaks in single‐stranded DNA and is thus a good method for determining the genotoxic potential of surface waters. In attempting to standardize the procedure, a wide range of factors which could have an influence on the uptake of genotoxic substances by the exposed clams were studied. The most important parameters of the static exposure in relation to the rate of filtration by the animals turned out to be the temperature, the volume of the water, and the exposure time. Differences in body size and in the amount of suspended particles in surface waters did not play a significant role. The results demonstrate that the in vivo test system can be quite sensitive and its results reproducible when the relevant species‐specific characteristics of the test organisms are brought into consideration, even if the test organism belongs to a biologically more advanced group. A clear dose‐response relationship to the reference substances 4‐nitroquinoline‐1‐oxide (NQO) and N‐methyl‐N‐nitro‐N‐nitrosoguanidine (MNNG) can be observed even after a short exposure time of between four and twenty hours. No genotoxic effects were observed when using this method on surface waters from the area of Cologne (including water from the Rhine River and within the protection zone 2 of the Cologne waterworks). http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Acta hydrochimica et hydrobiologica Wiley

Significance of an In Vivo Genotoxicity Test for Surface Waters Is Increased when the Physiological Characteristics of the Test Organism (Bivalvia: Mollusca) Are Taken into Account?

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Publisher
Wiley
Copyright
Copyright © 2000 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0323-4320
eISSN
1521-401X
DOI
10.1002/(SICI)1521-401X(20002)28:2<83::AID-AHEH83>3.0.CO;2-G
Publisher site
See Article on Publisher Site

Abstract

The alkaline filter elution assay using the gills of the freshwater clam species Corbicula fluminea detects breaks in single‐stranded DNA and is thus a good method for determining the genotoxic potential of surface waters. In attempting to standardize the procedure, a wide range of factors which could have an influence on the uptake of genotoxic substances by the exposed clams were studied. The most important parameters of the static exposure in relation to the rate of filtration by the animals turned out to be the temperature, the volume of the water, and the exposure time. Differences in body size and in the amount of suspended particles in surface waters did not play a significant role. The results demonstrate that the in vivo test system can be quite sensitive and its results reproducible when the relevant species‐specific characteristics of the test organisms are brought into consideration, even if the test organism belongs to a biologically more advanced group. A clear dose‐response relationship to the reference substances 4‐nitroquinoline‐1‐oxide (NQO) and N‐methyl‐N‐nitro‐N‐nitrosoguanidine (MNNG) can be observed even after a short exposure time of between four and twenty hours. No genotoxic effects were observed when using this method on surface waters from the area of Cologne (including water from the Rhine River and within the protection zone 2 of the Cologne waterworks).

Journal

Acta hydrochimica et hydrobiologica – Wiley

Published: Feb 1, 2000

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