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Sequential Immobilization of Enzymes in Microfluidic Channels for Cascade Reactions

Sequential Immobilization of Enzymes in Microfluidic Channels for Cascade Reactions Go with the flow: A flow‐through microfluidic reactor system was developed that contains the three enzymes, β‐galactosidase (β‐Gal), glucose oxidase (GOD), and peroxidase (HRP), immobilized with a polycationic dendronized polymer in a predetermined sequence on a single chip for the conduction of an enzymatic cascade reaction. The desired enzyme localization within the microchannels of the chip could be achieved with a micromechanical valve system. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png ChemPlusChem Wiley

Sequential Immobilization of Enzymes in Microfluidic Channels for Cascade Reactions

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References (30)

Publisher
Wiley
Copyright
Copyright © 2012 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim
ISSN
2192-6506
eISSN
2192-6506
DOI
10.1002/cplu.201100068
Publisher site
See Article on Publisher Site

Abstract

Go with the flow: A flow‐through microfluidic reactor system was developed that contains the three enzymes, β‐galactosidase (β‐Gal), glucose oxidase (GOD), and peroxidase (HRP), immobilized with a polycationic dendronized polymer in a predetermined sequence on a single chip for the conduction of an enzymatic cascade reaction. The desired enzyme localization within the microchannels of the chip could be achieved with a micromechanical valve system.

Journal

ChemPlusChemWiley

Published: Feb 1, 2012

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