Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Screening pigs for xenotransplantation: prevalence and expression of porcine endogenous retroviruses in G öttingen minipigs

Screening pigs for xenotransplantation: prevalence and expression of porcine endogenous... The group of Dr Denner at the Robert Koch Institute, Berlin‐FRG, reports in this issue of xenotransplantation on the presence of porcine endogenous retroviruses (PERVs) in the herd of Göttingen minipigs held at Ellegaard, Denmark . In a series of 15 animals all three subtypes, PERV‐A, PERV‐B and PERV‐C, were detected and also a new variant of PERV‐C that was recently described by the same group . The expression of PERV was higher than in other pig breeds analyzed by the same group and reported earlier elsewhere . This included German Landrace, Schwäbisch‐Hall, and Duroc/GL breeds: A small number of Large White pigs showed similar or even higher expression. Finally, blood mononuclear cells from five animals were stimulated with the mitogen phytohaemagglutinin and assessed for transmission of PERV into human embryonic kidney 293 cells, either by adding the supernatant of stimulated porcine cells to the human target cells or by coculture with separation of the two cell populations by a 0.4‐μm pore size membrane. In this in vitro transmission assay there was no PERV transmission detected. This manuscript is relevant for the discussion on safety of a xenotransplantation product for a number of aspects. The report is the http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Xenotransplantation Wiley

Screening pigs for xenotransplantation: prevalence and expression of porcine endogenous retroviruses in G öttingen minipigs

Xenotransplantation , Volume 20 (3) – May 1, 2013

Loading next page...
 
/lp/wiley/screening-pigs-for-xenotransplantation-prevalence-and-expression-of-aImSKv3FaC

References (22)

Publisher
Wiley
Copyright
© 2013 John Wiley & Sons A/S
ISSN
0908-665X
eISSN
1399-3089
DOI
10.1111/xen.12039
pmid
23611434
Publisher site
See Article on Publisher Site

Abstract

The group of Dr Denner at the Robert Koch Institute, Berlin‐FRG, reports in this issue of xenotransplantation on the presence of porcine endogenous retroviruses (PERVs) in the herd of Göttingen minipigs held at Ellegaard, Denmark . In a series of 15 animals all three subtypes, PERV‐A, PERV‐B and PERV‐C, were detected and also a new variant of PERV‐C that was recently described by the same group . The expression of PERV was higher than in other pig breeds analyzed by the same group and reported earlier elsewhere . This included German Landrace, Schwäbisch‐Hall, and Duroc/GL breeds: A small number of Large White pigs showed similar or even higher expression. Finally, blood mononuclear cells from five animals were stimulated with the mitogen phytohaemagglutinin and assessed for transmission of PERV into human embryonic kidney 293 cells, either by adding the supernatant of stimulated porcine cells to the human target cells or by coculture with separation of the two cell populations by a 0.4‐μm pore size membrane. In this in vitro transmission assay there was no PERV transmission detected. This manuscript is relevant for the discussion on safety of a xenotransplantation product for a number of aspects. The report is the

Journal

XenotransplantationWiley

Published: May 1, 2013

There are no references for this article.