Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Safe use of anti‐CD154 monoclonal antibody in pig islet xenotransplantation in monkeys

Safe use of anti‐CD154 monoclonal antibody in pig islet xenotransplantation in monkeys Anti‐CD154mAb is a powerful co‐stimulation blockade agent that is efficacious in preventing rejection, even in xenogeneic settings. It has been used in the majority of successful long‐term pig‐to‐non‐human primate islet transplantation models. However, its clinical use was halted as a result of thromboembolic complications that were also observed in preclinical and clinical organ transplantation models. An anti‐CD154mAb was administered to 14 streptozotocin‐induced diabetic cynomolgus monkey recipients of porcine islets, some of which received the agent for many months. Monkeys were monitored for complications, and blood monitoring was carried out frequently. After euthanasia, multiple biopsies of all organs were examined for histological features of thromboembolism. Anti‐CD154mAb prevented rejection of genetically engineered pig islets in all monkeys. No significant complications were attributable specifically to anti‐CD154mAb. There was no evidence of thromboembolism in multiple histological sections from all major organs, including the brain. Our data suggest that in diabetic monkeys with pig islet grafts, anti‐CD154mAb would appear to be an effective and safe therapy, and is not associated with thromboembolic complications. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Xenotransplantation Wiley

Safe use of anti‐CD154 monoclonal antibody in pig islet xenotransplantation in monkeys

Loading next page...
 
/lp/wiley/safe-use-of-anti-cd154-monoclonal-antibody-in-pig-islet-lVM0tRh2y4

References (37)

Publisher
Wiley
Copyright
© 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
ISSN
0908-665X
eISSN
1399-3089
DOI
10.1111/xen.12283
pmid
28058735
Publisher site
See Article on Publisher Site

Abstract

Anti‐CD154mAb is a powerful co‐stimulation blockade agent that is efficacious in preventing rejection, even in xenogeneic settings. It has been used in the majority of successful long‐term pig‐to‐non‐human primate islet transplantation models. However, its clinical use was halted as a result of thromboembolic complications that were also observed in preclinical and clinical organ transplantation models. An anti‐CD154mAb was administered to 14 streptozotocin‐induced diabetic cynomolgus monkey recipients of porcine islets, some of which received the agent for many months. Monkeys were monitored for complications, and blood monitoring was carried out frequently. After euthanasia, multiple biopsies of all organs were examined for histological features of thromboembolism. Anti‐CD154mAb prevented rejection of genetically engineered pig islets in all monkeys. No significant complications were attributable specifically to anti‐CD154mAb. There was no evidence of thromboembolism in multiple histological sections from all major organs, including the brain. Our data suggest that in diabetic monkeys with pig islet grafts, anti‐CD154mAb would appear to be an effective and safe therapy, and is not associated with thromboembolic complications.

Journal

XenotransplantationWiley

Published: Jan 1, 2017

Keywords: ; ; ;

There are no references for this article.