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Rice (Oryza sativa) Protoplast Isolation and Its Application for Transient Expression Analysis

Rice (Oryza sativa) Protoplast Isolation and Its Application for Transient Expression Analysis Rice (Oryza sativa) is not only the staple food for half of the world's population but also a model monocot plant for molecular biology studies. Although rice genes have been extensively investigated in the last two decades, the functions of many genes in the rice genome are still not known. One of the rapid and efficient approaches for determining gene function in vivo is protoplast‐based transient expression analysis. We established a rice protoplast system about 10 years ago, which has been recently used in many laboratories. This protocol is useful for protein expression, subcellular localization, bimolecular fluorescence complementation, and co‐immunoprecipitation assays. © 2016 by John Wiley & Sons, Inc. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Current Protocols in Plant Biology Wiley

Rice (Oryza sativa) Protoplast Isolation and Its Application for Transient Expression Analysis

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References (20)

Publisher
Wiley
Copyright
© John Wiley and Sons
ISSN
2379-8068
eISSN
2379-8068
DOI
10.1002/cppb.20026
Publisher site
See Article on Publisher Site

Abstract

Rice (Oryza sativa) is not only the staple food for half of the world's population but also a model monocot plant for molecular biology studies. Although rice genes have been extensively investigated in the last two decades, the functions of many genes in the rice genome are still not known. One of the rapid and efficient approaches for determining gene function in vivo is protoplast‐based transient expression analysis. We established a rice protoplast system about 10 years ago, which has been recently used in many laboratories. This protocol is useful for protein expression, subcellular localization, bimolecular fluorescence complementation, and co‐immunoprecipitation assays. © 2016 by John Wiley & Sons, Inc.

Journal

Current Protocols in Plant BiologyWiley

Published: Mar 1, 2016

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