Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Requirement of CD4 cells for induction and maintenance of unresponsiveness in islet xenografted mice

Requirement of CD4 cells for induction and maintenance of unresponsiveness in islet xenografted mice Abstract: Long‐term survival of islet xenografts in the hamster to mouse model can be induced by a short‐course treatment with a nondepleting anti‐CD4 mAb but not with a depleting anti‐CD4 mAb (Lu et al. Xenotransplantation 1998; 5:154–1631. Although CD4 cells are known to play a key role in the rejection of islet xenografts, it remains unclear whether CD4 cells are also required for the induction and/or maintenance of specific unresponsiveness to xenografts. To investigate this problem, islets were isolated from golden hamsters and transplanted into streptozotocin‐induced diabetic CBA/J mice. Nondepleting mAb YTS 177.9 was used to block CD4 cells for the induction of islet xenograft unresponsiveness and subsequently depleting mAb GK1.5 to deplete CD4 cells in the unresponsive recipients. First, we now confirm that second donor‐strain xenografts were permanently accepted in recipients that had been unresponsive to the first grafts, whereas Lewis rat islet xenografts, used as third‐party grafts, were rejected like a primary graft within 7–8 days. Second, we depleted CD4 cells in recipient mice, which had been treated perioperatively with the nondepleting mAb YTS 177.9 and became unresponsive to their primary hamster islet graft, by using a depleting anti‐CD4 mAb at different time points post‐transplant. Depletion of CD4 cells in the unresponsive recipients by the depleting anti‐CD4 mAb GK1.5 did abrogate this unresponsive state, since the grafts were always rejected within an average of 25.5 days after the mAb GK1.5 injections. Therefore, our results strongly suggest that CD4 positive cells play an active suppressive role and that their presence in the recipients appears essential for both induction and maintenance of long‐term islet xenograft survival or specific unresponsiveness. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Xenotransplantation Wiley

Requirement of CD4 cells for induction and maintenance of unresponsiveness in islet xenografted mice

Lu, Xiaoshan; Borel, Jean F.
Xenotransplantation , Volume 5 (3) – Aug 1, 1998
Download PDF
8 pages

Loading next page...
 
/lp/wiley/requirement-of-cd4-cells-for-induction-and-maintenance-of-Ss40tBWuLh

References (41)

Publisher
Wiley
Copyright
Copyright © 1998 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0908-665X
eISSN
1399-3089
DOI
10.1111/j.1399-3089.1998.tb00029.x
Publisher site
See Article on Publisher Site

Abstract

Abstract: Long‐term survival of islet xenografts in the hamster to mouse model can be induced by a short‐course treatment with a nondepleting anti‐CD4 mAb but not with a depleting anti‐CD4 mAb (Lu et al. Xenotransplantation 1998; 5:154–1631. Although CD4 cells are known to play a key role in the rejection of islet xenografts, it remains unclear whether CD4 cells are also required for the induction and/or maintenance of specific unresponsiveness to xenografts. To investigate this problem, islets were isolated from golden hamsters and transplanted into streptozotocin‐induced diabetic CBA/J mice. Nondepleting mAb YTS 177.9 was used to block CD4 cells for the induction of islet xenograft unresponsiveness and subsequently depleting mAb GK1.5 to deplete CD4 cells in the unresponsive recipients. First, we now confirm that second donor‐strain xenografts were permanently accepted in recipients that had been unresponsive to the first grafts, whereas Lewis rat islet xenografts, used as third‐party grafts, were rejected like a primary graft within 7–8 days. Second, we depleted CD4 cells in recipient mice, which had been treated perioperatively with the nondepleting mAb YTS 177.9 and became unresponsive to their primary hamster islet graft, by using a depleting anti‐CD4 mAb at different time points post‐transplant. Depletion of CD4 cells in the unresponsive recipients by the depleting anti‐CD4 mAb GK1.5 did abrogate this unresponsive state, since the grafts were always rejected within an average of 25.5 days after the mAb GK1.5 injections. Therefore, our results strongly suggest that CD4 positive cells play an active suppressive role and that their presence in the recipients appears essential for both induction and maintenance of long‐term islet xenograft survival or specific unresponsiveness.

Journal

Xenotransplantation – Wiley

Published: Aug 1, 1998

There are no references for this article.